Similarity Check Comparison of Specthrophotometric and TLC-Densitometric Technique in Determination of Phytomelatonin in Green Algae (Sprirogyra Sp) Ethanolic Extract

Susanti, Hari and Widyaningsih, Wahyu and Salamah, Nina and Zudla Fertaveni, Beta and Puspitasari, Efi Similarity Check Comparison of Specthrophotometric and TLC-Densitometric Technique in Determination of Phytomelatonin in Green Algae (Sprirogyra Sp) Ethanolic Extract. Fakultas Farmasi, Universitas Ahmad Dahlan.

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Abstract

Background. Instrumental analysis method give a difference measurement results in determination of content level of active substance in medicinal herb. Objective. The aim of this study is to campare the level content of phytomelatonin in green algae extract determinated by spectrophotometry and TLC-densitometry technique. Methods. The phytomelatonin was extracted from green algae using 96% aethanol. The qualitative Alkaloid screening were done by using Mayer and Dragendorff test. The quantitative determination of phytomelatonin were done spectrophotometrically at 277nm wavelength and using TLC densitometry technique with silica gel GF 254 plate and eluated by BAW ( n-buthanol: acetic acid: water=12:3:5 v/v). The spots were scanned at 254nm wavelength Outcome measured. Phytomelatonin level in aethanolic extract of green algae Results. The results showed that the content phytomelonin content of green algae level by spectrophotometric technique was 0.22±0.01 % and 0.88±0.04 % was assayed by TLC-densitometric. Conclusion. The TLC-densitometry technique gave the higher phytomelatonin of green algae level than specthrophotometry technique (p<0.05)

Item Type: Other
Subjects: Q Science > QK Botany
R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica
Divisi / Prodi: Faculty of Pharmacy (Fakultas Farmasi) > Program of Professional Pharmacist
Depositing User: Mrs Hari Susanti
Date Deposited: 01 Oct 2020 04:06
Last Modified: 01 Oct 2020 04:06
URI: http://eprints.uad.ac.id/id/eprint/20700

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