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Comparasion Of Specthrophotometric And TCL-Densitometric Technique In Determination Of Phytomelatonin In Green Algae (Sprirogyra sp) Ethanolic Extract

Susanti, Hari and Widyaningsih, Wahyu and Salamah , Nina and Zudia Fertaveni, Beta and Puspitasari, Efi (2013) Comparasion Of Specthrophotometric And TCL-Densitometric Technique In Determination Of Phytomelatonin In Green Algae (Sprirogyra sp) Ethanolic Extract. Proceeding The International Conference on Safety Management Of Central Cytotoxic Reconstitution In Pharmacy Practice. pp. 191-196. ISSN 978-979-18458-6-1

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Abstract

Bacground. Instrumental analysis method give a difference measurement results in determination of content level of active substance in medicinal herb. Objective.The aim of this study is to compare the level content of phytomelatonin in gren algae extract determinated by spectrophotometry and TCL-densitometry technique. Methods. the phytomelatonin was extracted from green algae using 90%aethanol. The qualitative Alkaloid screening were done by using Mayer and Dragendroff test. The quantitative determination of phytomelatonin were done spectrophotometrically at 277nm wavelength and using TCL densitometrt techinique with silica gel GF 254 plate and eluated by BAW (n-buthanol:acetic acid: water=12:3:5 v/v). The spots were scanned at 254nm wavelength. Outcome measured. Phytomelatonin level in aethonolic extract of green algae Results. The result showed that the content phytomelonin content of green algae level by spectrophotometric technique was 0.22+-0.01 % and 0.88+_0.04% was assayed by TCL-desitometric. Conclusion. the TCL-desitometry technique gave the higher phytomelatonin of green algae level than specthrophometry technique (p<0.05) Keywords: Phytomelatonin, green algae, aethanolic exctract, spectrophotometry, TCL-densitometry.

Item Type: Article
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User: Ms Nina Salamah
Date Deposited: 21 Oct 2015 03:13
Last Modified: 21 Oct 2015 03:13
URI: http://eprints.uad.ac.id/id/eprint/2474

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