Ethanolic Extract Of Pasak Bumi Leaves Antiangiogenic Activity On Chorioallantoic Membrane Of Chicken Embryo Induced By bFGF

Salamah , Nina (2012) Ethanolic Extract Of Pasak Bumi Leaves Antiangiogenic Activity On Chorioallantoic Membrane Of Chicken Embryo Induced By bFGF. Proceeding Of international Conference On Drug Delevopment Of Natural Recources. pp. 111-117. ISSN 978-979-18458-5-4

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Abstract

Bacground. Angiogenesis, the growth of the blood vessel,allows cells to get enough supply oxygen and nutrient.Some study on natural substances have been counducted including pasak bumi leaves (Eurycoma longifolia,Jack)as anticancer. The aim of this study was to know the antiangiogenic effect of pasak bumi leaves using CAM method.
Method. The ethanolic extract of pasak bumi leaves was prepared using maceration methode. The 9 day incubated chicken embryo were divided into seven groups.The group I was the control group received paper disc,group II was the bFGF controller,group III was the bFGF controller and DMSO 0,8% as the solvent.The test groupreceived different concentration (65 ug/ml,130 ug/ml,260 ug/ml, and 520 ug/ml) of extract ethanol of pasak bumi leaf in combination with 10 ug bFGF. After having been incubated for theee days, the content in the egg was taken out.Then, the corio allantoic membrane attaching the eggshell was carefully observed macroscopically and microscopically. The result of macroscopically observation was quantified and analyzed.
Result. Based on the result of the study, the ethanol extract of pasak bumi leaves can actually inhibited angiogenesis on CAM induced by bFGF.
Conclusion. Ethanol extract of pasak bumi leaves shoewd antiangiogenetic activity began to level 130 mg/ml and increasing concentrations of ethanol extract of pasak bumi leaves enchance angiogenesis inhibitory activity.

Keywords: angiogenesis, Eurycoma longifolia, pasak bumi, CAM, bFGF

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	Ms Nina Salamah
Date Deposited:	21 Oct 2015 03:13
Last Modified:	21 Oct 2015 03:13
URI:	http://eprints.uad.ac.id/id/eprint/2475

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