Identifying active compounds of soursop ethanolic fraction as α-glucosidase inhibitor

Mugiyanto, Eko and Cahyanta, Agung Nur and Putra, I Made Agus Sunadi and Setyahadi, Siswa and Simanjuntak, Partomuan (2019) Identifying active compounds of soursop ethanolic fraction as α-glucosidase inhibitor. Pharmaciana, 9 (2). pp. 191-200.

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Abstract

Postprandial hyperglycemia is triggered by two enteric proteins (α-amylase and α-glucosidase) connected to the brush border of intestinal cells. In diabetic populations, Type 2 Diabetes Mellitus is more prevalent, and α-glucosidase inhibition is the suitable therapy for delaying glucose intake after meals. This study was designed to investigate chemical compounds for their potential inhibitory effects on α-glucosidase. It employed a spectrophotometric method with p-nitrophenyl-α-D-glucopyranose from Saccharomyces cerevisiae as an alpha-glucosidase substrate. The ethanolic fraction was acquired from assay- or activity-guided fractionation. The findings showed that the ethanolic fraction of soursop leaves exhibited the most significant inhibitory activity with an IC50 of 0.17 μg/ml. The data analysis and active compounds identification were carried out by LC-MS and FT-IR. The active compounds of the resulted mixture comprised of Muricatin C, cis-Reticulatacin-10-one, and 3-Methylquercetin 7-[galactosyl-(1->4)-glucoside].

Item Type: Artikel Umum
Subjects: R Medicine > RS Pharmacy and materia medica
Divisi / Prodi: Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User: jurnal pharmaciana
Date Deposited: 29 Sep 2021 21:18
Last Modified: 29 Sep 2021 21:18
URI: http://eprints.uad.ac.id/id/eprint/28736

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