Peer Review In Vitro Antioxidant Activity of Lampes (Ocimum sanctum) Leaves and Seeds Ethanol Extract Using DPPH Method

Susanti, Hari Peer Review In Vitro Antioxidant Activity of Lampes (Ocimum sanctum) Leaves and Seeds Ethanol Extract Using DPPH Method. Fakultas Farmasi UAD.

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Abstract

Background. Recent study reported that polyphenol compounds have a large contributionin
antioxidant activity. Polyphenol compound was reported as one of the content of Ocimum sanctum.L
The aim of this study was to observe the antioxidant activity of lampes (Ocimum sanctum) leaves and
seeds ethanol extract using DPPH method.
Method. The extraction was done using maceration method. The antioxidant activity was
measured byDPPH method and gallic acid was used as comparative substance. Total phenolic
content of ethanol extract was determinated by spectrophotometric method with Folin-Ciocalteu
reagent quantitatively. Furthermore, the effective Scavenging 50 (ES50) value was calculated using
linear regression between ethanol extract or galic acid concentration versus % of free radical
scavenging. The ES50 values were statistically analysis with anova and LSDTest.
Result.This study showed that ethanolextract of Ocimum sanctum seeds has antioxidant
potency with ES50 value was 131.81 ìg/ml> ES50 of leaves (91.94 ìg/ml ) > ES 50of of gallic acid (
3.54 ìg/ml)( p<0.05).The total phenolic content of ethanolextract of Ocimum sanctumleaves was
41.33 mg/gram, while the phenolic content of etanolic extract of seeds was 26.81 mg/gram wich was
expressed as gallic acid equivalent (GAE).

Item Type:	Other
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General) T Technology > T Technology (General)
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > Pharmacy Doc
Depositing User:	Mrs Hari Susanti
Date Deposited:	21 May 2022 01:16
Last Modified:	26 Sep 2022 03:57
URI:	http://eprints.uad.ac.id/id/eprint/32361

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