Black cumin seed oil increase leucocyte and CD4Thelper number in sprague-dawley rats induced with dimethylbenzanthracene

Akrom, Akrom and Hidayati, Titiek and Sagiran, Sagiran and Indrayanti, Indrayanti (2019) Black cumin seed oil increase leucocyte and CD4Thelper number in sprague-dawley rats induced with dimethylbenzanthracene. International Journal of Public Health Science (IJPHS), 8 (2). ISSN 2252-8806

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Abstract

Cigarette smoke contains 7, 12 dimethylbenzanthracene (DMBA). Metabolic
of DMBA is immunosuppressive. Black cumin seed oil (BCSO) is
immunomodulation. This study aimed to determine the effect of BCSO on
leukocyte, CD4Th and CD4CD25Treg in Sprague-Dawley (SD) mice
induced with DMBA. The 96 SD rats were divided into eight groups of 12.
Group I received aquabidest and regular feeding. Groups II, III and IV
received BCSO (an equivalent of 6.8, 68 and 136 mg/kg BW/day
thymoquinone, respectively). Group V received thymoquinone
(50 mg/kg BW/day) and group VI received tamoxifen (60 mg/kg BW).
Group VII (DMBA) was induced with DMBA (10x20mg/kg BW for five
weeks). Group VIII received regular feeding and corn oil treatment. In the
third week, all groups began to be induced with DMBA (20 mg/kg BW twice
per week for five weeks). Data collection of leukocytes, CD4Th and
CD4CD25Treg, were performed at week 27th. The mean difference of
CD4Th and CD4CD25Treg counts between groups was calculated with one
way ANOVA. The administration of BCSO, thymoquinone, and tamoxifen
had increased leukocytes and CD4 Th cell count. The CD4Th cell count of
the treatment groups was higher than that of the DMBA group (p<0.05).
BCSO equivalent doses of 6.8 and 68 mg/kg BW/day thymoquinone showed
immunoprotective effects. It can be concluded that the BCSO administration
at doses of 6.8 and 68 mg/kg B/day shows immunoprotective effects due to
DMBA induction.

Item Type:	Artikel Umum
Subjects:	Z Bibliography. Library Science. Information Resources > Z665 Library Science. Information Science
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	Akrom Akrom
Date Deposited:	21 Jun 2022 01:44
Last Modified:	21 Jun 2022 01:44
URI:	http://eprints.uad.ac.id/id/eprint/35445

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