THE EFFECT OF COFFEE FRUIT SKIN EXTRACT ON SPERM CHARACTERISTICS AND TESTICULAR OF MICE WITH ETHANOL-INDUCED

Setiawan, haris (2020) THE EFFECT OF COFFEE FRUIT SKIN EXTRACT ON SPERM CHARACTERISTICS AND TESTICULAR OF MICE WITH ETHANOL-INDUCED. [Artikel Dosen]

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Abstract

Coffee fruit skin contains antioxidant compounds that can repair damaged tissues, especially those of reproductive or-gans. This study was designed to assess to what extent the metha-nolic extract of coffee fruit skin affected sperm characteristics and testicular histology in mice receiving 15% ethanol for 15 days. It used 25 male mice aged four months and employed a completely randomized design with two controls (K) and three treatments (P), namely K1 (without ethanol), K2 (15% ethanol), P1 (15% ethanol and the methanolic extract of coffee fruit skin, or MECS, at 125 mg/ kg BW), P2 (15% ethanol and MECS 250 mg/kg BW), and P3 (15% ethanol and MECS 500 mg/kg BW). These treatments were administered orally with a 1 mL disposable syringe for 15 days. The parameters observed were sperm characteristics (viability, morphology, and sperm count) and the microscopic structure of the testicles (lu-men area, diameter, and area of seminiferous tubules). These data were then analyzed using the one-way ANOVA, continued with the LSD and Duncan’s tests (P <0.05). The results showed that P2 (250 mg/kg BW) substantially improved sperm count (86.5x105±1.73 cells/ mL), sperm viability (87.26±0.05%), and the proportion of normal sperm morphology (93.33±0.004%) of mice with 15% ethanol-in-duced sperm damages. Testicular histology also confirmed improve-ments in spermatogenic cells, as evident from the lumen, diameter, and area seminiferous tubules after receiving P1 and P2. In conclu-sion, the optimum dose of the coffee skin extract for improving sperm quality and microscopic structures of mice testicles is 250 mg/kg BW.

Item Type:	Artikel Dosen
Subjects:	Q Science > QL Zoology
Divisi / Prodi:	Faculty of Applied Science and Technology (Fakultas Sains Dan Teknologi Terapan) > S1-Biology (S1-Biologi)
Depositing User:	M.Sc. Haris Setiawan
Date Deposited:	13 Sep 2022 02:31
Last Modified:	13 Sep 2022 02:31
URI:	http://eprints.uad.ac.id/id/eprint/36846

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