High Keratin Secretion of T47D Cell under Hypoxic Condition

Annisa, Annisa (2022) High Keratin Secretion of T47D Cell under Hypoxic Condition. [Artikel Dosen]

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	Text SECRETORY PROTEIN-RELATED HYPOXIA IN T47D BREAST CANCER CELL LINE.pdf Download (2MB)

Abstract

BACKGROUND: Hypoxia adaptation in cancer
cells is mediated by hypoxia inducible factor (HIF)-
1 that induces the expression of various proteins.
Up to date, the analysis of T47D breast cancer cells-secreted
protein is still limited. The aim of this study was to compare
the protein profile secreted by T47D breast cancer cells
under hypoxic and to normoxic conditions and identify the
proteins as candidate for hypoxia marker proteins in T47D
breast cancer cells.
METHODS: T47D breast cancer cells were cultured under
standard conditions. Cells were subcultured in normoxic
and hypoxic conditions. The normoxic group was incubated
with 20% oxygen and the hypoxic groups were incubated
in a hypoxic chamber with 0.5% and 5% oxygen for 6, 24,
and 48 hours in serum free medium. Proteins in the culture
media were isolated and precipitated. Protein concentrations
released by the cells were then measured by bicinchoninic
assay (BCA). Protein bands were visualized by the sodium
dodecyl sulfate polyacrylamide gel electrophoresis (SDS�PAGE) method. The bands that had differences between
the hypoxic and normoxic groups were further analyzed by
liquid chromatography-mass spectrophotometer (LC-MS).
RESULTS: Hypoxic groups showed higher secretory
protein than normoxic group. Protein bands were found in
the 0.5% hypoxic group with a size of 50-75 kDa. Secretory
proteins identified by LC-MS were keratin 1, 2, 9, and 10.
CONCLUSION: The T47D cell line under 0.5% hypoxic
treatment showed higher secretory proteins that identified
as keratin 1, 2, 9, and 10.
KEYWORDS: hypoxia, secretory protein, liquid
chromatography-mass spectrophotometer
Indones Biomed J. 2022; in press

Item Type:	Artikel Dosen
Subjects:	R Medicine > R Medicine (General)
Divisi / Prodi:	Fakultas Kedokteran > S1 - Kedokteran
Depositing User:	dr. Annisa Annisa
Date Deposited:	05 Oct 2022 01:48
Last Modified:	14 Dec 2022 07:59
URI:	http://eprints.uad.ac.id/id/eprint/37209

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