Hasil cek similarity" Isolation of active compound from Nephelium lappaceum L. rind as an antioxidant"

Nurani, Laela Hayu and Edityaningrum, Citra Ariani and Suhendra, Suhendra and Windarsih, Anjar and Riyanto, S and Rohman, Abdul (2022) Hasil cek similarity" Isolation of active compound from Nephelium lappaceum L. rind as an antioxidant". Food Research, 6 (3).

Text HASIL CEK_Isolation of active compound from Napolium lappaceum L rind as an antioxidant_Food Research.pdf Download (2MB)

Abstract

The consumption of rambutan (Nephelium lappaceum L.) results in a vast amount of
rambutan rind. It is very interesting to explore rambutan rind as natural antioxidants. This
study aimed to explore the antioxidant activities of rambutan rind in vitro and to correlate
those activities with a group of compounds (phenolics and flavonoids). Rambutan rind
was cleaned, dried using a conventional oven, powdered, and the powder obtained was
subjected to maceration. The macerate was fractionated using petroleum ether and
chloroform to get chloroform fraction. The sub fractionation was done toward chloroform
fraction. The chloroform fraction and its subfractions were then subjected to antioxidant
activity measurement, determination of phenolics and flavonoid contents, and the active
compound present in the most active subfractions was isolated using column
chromatography. The isolate was identified using several methods including thin-layer
chromatography (TLC), Fourier transform infrared (FTIR) spectroscopy as well as gas
chromatography-mass spectrometry (GC-MS). The results showed that the chloroform
fraction and its subfractions were correlated with the phenolic and flavonoid contents
found in rambutan rinds. The phenolic and flavonoid content of chloroform fraction was
32.32±0.79 and 55.05±3.900, respectively, whereas the IC50 of antioxidant activity was
49.77±0.22 for DPPH and 45.36±0.27 for ABTS. The reducing power was 34.478±0.245
(mg vitamin C equivalent/g sample) and the metal chelating activity was 332.753±0.695
(mg Na EDTA equivalent/g sample). The phenolic contents contributed towards the metal
chelating activities compared to other antioxidant activities with an R
2
value of 0.8726,
while flavonoid contents contributed more to ABTS radical scavenging activity with an R
2
value of 0.8916. One of the active compounds present in rambutan rind having antioxidant
activity was identified as 1,2-benzenedicarboxylic acid. Thus, the under-utilized part of
rambutan rind could be explored as a natural antioxidant to be used as a food supplement

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi)
Depositing User:	Laela
Date Deposited:	04 Nov 2022 01:26
Last Modified:	04 Nov 2022 01:26
URI:	http://eprints.uad.ac.id/id/eprint/37427

Actions (login required)

View Item