Hasil cek similarity" Potentially Virulent Multi‐Drug Resistant Escherichia fergusonii Isolated from Inanimate Surface in a Medical University: Omphisa fuscidentalis as an Alternative for Bacterial Virulence Determination"

Ilsan, Noor Andryan and Yunita, Melda and Dewi, Nurul Kusuma and Irham, Lalu Muhammad and Sipriyadi, Sipriyadi and Nurfajriah, Siti and Inggraini, Maulin Hasil cek similarity" Potentially Virulent Multi‐Drug Resistant Escherichia fergusonii Isolated from Inanimate Surface in a Medical University: Omphisa fuscidentalis as an Alternative for Bacterial Virulence Determination". Diagnostics.

Text Hasil cek_Potentially Virulent Multi‐Drug Resistant Escherichia fergusonii Isolated from Inanimate Surface in a Medical University_ Omphisa fuscidentalis as an Alternative for Bacterial Virulence Determination.pdf Download (3MB)

Abstract

Abstract: Multi‐drug resistant (MDR) bacteria are becoming a worldwide problem due to limited
options for treatment. Moreover, patients infected by MDR with highly virulent accessories are
worsening the symptoms, even to the point of causing death. In this study, we isolated bacteria from
14 inanimate surfaces that could potentially be reservoirs for the spread of bacterial infections in the
medical university. Blood agar media was used for bacterial isolation. The bacterial colony that
showed hemolytic activities on each surface was tested for antimicrobial susceptibility against eight
different antibiotics. We found that MDR bacterium, namely TB1, which was isolated from a toilet
bowl, was non‐susceptible to ampicillin, imipenem, chloramphenicol, amoxicillin‐clavulanic acid,
gentamicin, and tetracycline. Another MDR bacterium isolated from the mobile phone screen of
security officers, namely HSO, was resistant to chloramphenicol, gentamicin, tetracycline, and cefixime.
An in vivo virulence test of bacterial isolates used Omphisa fuscidentalis larvae as an alternative
to Galleria mellonella larvae for the infection model. A virulence test of TB1 in O. fuscidentalis larvae
revealed 20% survival in the bacterial density of 104 and 105 CFU/larvae; and 0% survival in the
bacterial density of 106 CFU/larvae at 24 h after injection. Bacterial identification was performed for
TB1 as a potential virulent isolate. Bacterial identification using partial 16s rRNA gene showed that
TB1 exhibited 99.84% identity to Escherichia fergusonii 2611. This study concludes that TB1 is a potentially
virulent MDR E. fergusonii isolated from toilet bowls at a medical university.

Item Type:	Artikel Umum
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi)
Depositing User:	Lalu Muhammad Irham, M.Farm., Ph.D
Date Deposited:	16 Jan 2023 02:56
Last Modified:	16 Jan 2023 02:56
URI:	http://eprints.uad.ac.id/id/eprint/38831

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