Validation of rhodamin b analysis method in beef sausage with visible spectrophotometry instruments

Kurniaty, Santyas and Salamah, Nina Validation of rhodamin b analysis method in beef sausage with visible spectrophotometry instruments. JOURNAL OF HALAL SCIENCE AND RESEARCH, 4 (1). ISSN 2715-6214

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Abstract

Sausage is made from meat that has been chopped and then mashed, seasoned, and put in a symmetrical elliptical sleeve, made either from animal intestines or artificial wrapping (casing). To attract consumer interest, synthetic food coloring such as Rhodamin B is added. Rhodamin B is a dye prohibited from being used in products, according to the Director General of POM No. 00386/C/SK/II/1990, because the use of Rhodamin B for a long time can cause liver damage and cause cancer. The purpose of this study was to validate the analytical method of rhodamine B in beef sausages by visible spectrophotometry. The sample used in this study was a beef sausage with a reddish-pink characteristic and without a brand. The validation parameters of the method used in this study are linearity, precision, LOD, LOQ, and accuracy. The data obtained were then analyzed using the SPSS Kolmogorov-Smirnov method, then ANOVA was used. The validation results of linearity test methods, LOD LOQ, precision, and accuracy met the requirements of PPOP GMP in 2013.

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	Ms Nina Salamah
Date Deposited:	20 Feb 2023 04:14
Last Modified:	20 Feb 2023 04:14
URI:	http://eprints.uad.ac.id/id/eprint/40009

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