Separation of Polar Compounds using Poly(Ethylene Oxide) Bonded Stationary Capillary Liquid Chromatography

Linda, Rosa and ABDULLAH, ABDULLAH and Rafi, Mohamad and Rahayu, Aster and Lim, Lee Wah and Takeuchi, TOYOHIDE (2021) Separation of Polar Compounds using Poly(Ethylene Oxide) Bonded Stationary Capillary Liquid Chromatography. Sains Malaysiana, 50 (11). pp. 3241-3250. ISSN 0126-6039

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Abstract

Poly(ethylene oxide) (PEO) bonded stationary phase has been synthesized by a single and simple step reaction.
Poly(ethylene glycol monomethyl ether p-toluene sulfonate) (tosylated-PEO, molecular weight 900, n ≈ 18) was
chemically bonded to 3-aminopropyl silica (TSKgel NH2
-60, 5 µm particle size, and 60 Å mean pore diameter). The
prepared stationary phase was able to separate polar compounds such as phenolics and nucleobases in capillary
liquid chromatography. The retention and separation of phenolics and nucleobases could be achieved under isocratic
elution condition. Nucleobases such as thymine, adenine, uracil, uridine, cytidine and toluene and phenolics (phenol,
pyrocatechol, pyrogallol) were baseline separated in less than 6 min using 98% acetonitrile and less than 7 minutes
using 80% acetonitrile, respectively. We demonstrated that the retention of nucleobases as analyte decreased with
decreasing eluent concentration. The retention of these polar compounds was believed to be based on dipole-dipole
and/or hydrogen-bonding interactions.
Keywords: Capillary liquid chromatography; poly(ethylene oxide); polar compounds

Item Type:	Artikel Umum
Keyword:	Capillary liquid chromatography; poly(ethylene oxide); polar compounds
Subjects:	Q Science > QD Chemistry
Divisi / Prodi:	Faculty of Industrial Technology (Fakultas Teknologi Industri) > S1-Chemical Engineering (S1-Teknik Kimia)
Depositing User:	Ph.D Aster Rahayu
Date Deposited:	31 Mar 2023 01:57
Last Modified:	31 Mar 2023 01:57
URI:	http://eprints.uad.ac.id/id/eprint/41906

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