Antibacterial activity of ethanol extracts of Hibiscus tiliaceus L. leaves from different extraction methods against Escherichia coli and Staphylococcus aureus

Hidayati, Devi Nisa and Maghfiroh, Hanik Rohmatul and Safitri, Aysah (2023) Antibacterial activity of ethanol extracts of Hibiscus tiliaceus L. leaves from different extraction methods against Escherichia coli and Staphylococcus aureus. Pharmaciana, 13 (1). pp. 137-145. ISSN ISSN:2088-4559,e-ISSN:2477-0256

[thumbnail of 14.Nisa hidayati_137-145.pdf] Text
14.Nisa hidayati_137-145.pdf
Download (584kB)

Abstract

The leaves of sea hibiscus (Hibiscus tiliaceus L.) are known to have good antibacterial activity against Escherichia coli and Staphylococcus aureus, which is associated with the saponin, flavonoid, polyphenol, and tannin contents. Chemical compounds in plant extracts are, however, influenced by the extraction method used. This study aimed to determine the antibacterial activity of the ethanol extracts of Hibiscus tiliaceus L. leaves obtained using four different extraction methods against E. coli and S. aureus. In the maceration, percolation, reflux, or Soxhlet method, 200 g of the crude drug (i.e., dried sea hibiscus leaf powder) was extracted with 96% ethanol, and the derived extract was tested at 1%, 2%, 3%, 4%, and 5% concentrations. The disk diffusion method was used to test the antibacterial activity by observing and statistically analyzing the diameter of the zones of inhibition. Results showed that all the ethanol extracts have antibacterial properties against E. coli and S. aureus, with the maceration-produced extract forming significantly different zones of inhibition from the other methods (percolation, reflux, and the Soxhlet) at all concentrations.

Item Type: Artikel Umum
Subjects: R Medicine > RS Pharmacy and materia medica
Divisi / Prodi: Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User: jurnal pharmaciana
Date Deposited: 15 Apr 2023 03:58
Last Modified: 15 Apr 2023 03:58
URI: http://eprints.uad.ac.id/id/eprint/42846

Actions (login required)

View Item View Item