Antibacterial activities of Elaeocarpus ganitrus Roxb. leaves from Kebumen against Escherichia coli and Shigella flexneri

Kiromah, Naelaz Zukhruf Wakhidatul and Fitriyati, Laeli Fitriyati and Septiani, Sinta Wahyu (2023) Antibacterial activities of Elaeocarpus ganitrus Roxb. leaves from Kebumen against Escherichia coli and Shigella flexneri. Pharmaciana, 13 (2). pp. 232-239. ISSN ISSN:2088-4559,e-ISSN:2477-0256

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Abstract

Ganitri (Elaeocarpus ganitrus Roxb) is a plant known for its antibacterial properties, particularly in
its leaves. The leaves of Ganitri contain antibacterial compounds such as flavonoids, alkaloids, and
tannins. The objective of this study is to investigate the antibacterial activity and determine the
minimum concentration of ethanol, methanol, and distilled water extracts from Ganitri leaves in
inhibiting the growth of Escherichia coli and Shigella flexneri bacteria. A preliminary study of
phytochemical with Thin Layer Chromatogram (TLC) and antibacterial study was conducted. The
study was conducted in vitro by disc diffusion method with positive control of ciprofloxacin 50 μg/mL
and negative control of sterile aquadest. The extract concentration series used were 10, 20, 30, 40, 50,
and 100%. The extracts of ganitri leaves have antibacterial activities against Escherichia coli and
Shigella flexneri bacteria. The TLC results were observed under visible light at 254 and 365 nm.
Ethanol, methanol, and aquadest extracts of ganitri leaves produce spots that are almost comparable to
the compounds of quercetin and tannic acid. The inhibitory zone range of the extracts in Escherichia
coli bacteria is 10.33-19.66 mm with a strong category. In comparison, the inhibitory zone range of
Shigella flexneri is 8.88-22.62 mm with a moderate-very strong category. ANOVA test results stated a
significance value of p = 0.001 (p <0.05). Therefore, it can be concluded that the ethanol, methanol,
and aquadest extracts of ganitri leaves had significant differences. The minimum concentration of each
extract in inhibiting the bacteria Escherichia coli and Shigella flexneri is 10%.

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	jurnal pharmaciana
Date Deposited:	19 Aug 2023 03:49
Last Modified:	19 Aug 2023 03:49
URI:	http://eprints.uad.ac.id/id/eprint/46436

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