Effect of ethanol solvent concentrations in pepino melon fruit (Solanum muricatum Aiton) extraction on total flavonoid, phenolic, and β-carotene content

Firsty, Girly Risma and Sugihartin, Sugihartini and Mulyaningsih, Sri (2023) Effect of ethanol solvent concentrations in pepino melon fruit (Solanum muricatum Aiton) extraction on total flavonoid, phenolic, and β-carotene content. Pharmaciana, 13 (2). pp. 257-267. ISSN ISSN:2088-4559,e-ISSN:2477-0256

Text 13.Sri_257-267.pdf Download (616kB)

Abstract

The quality of an extract is determined by the type and level of compounds contained therein. The
solvent is one of the elements that influences the extract's quality. Therefore, extraction must be
carried out using a solvent that can extract bioactive substances. This study was aimed at obtaining the
optimal concentration of ethanol in extracting flavonoids, phenolics, and β-carotene from pepino
melon fruit. In this study, dried pepino melon fruit was extracted using ethanol of 50%, 70%, and 96%.
The extracts were analyzed qualitatively for phenolic and flavonoid using thin layer chromatography
(TLC), and quantitatively using a spectrophotometry. Meanwhile, β-carotene levels were determined
using high pressure liquid chromatography (HPLC). The data of level flavonoid, phenolic, and β-
carotene were statistically analyzed using multivariate analysis of variance. The results showed that
50%, 70%, and 96% ethanol solvent produced extracts with a yield value of 51.8%; 87.4%; 54.6%;
total flavonoid content of 0.298 ± 0.04 mgQE/g; 0.559 ± 0.03 mgQE/g; 0.289 ± 0.01 mgQE/g; total
phenolic content of 4.763 ± 0.08 mgGAE/g; 3.631 ± 0.12 mgGAE/g; 3.317 ± 0.10 mgGAE/g; β-
carotene level of 0.157 ± 0.02 mg/g; 0.910 ± 0.16 mg/g; 1.054 ± 0.13 mg/g, respectively. The
statistical analysis showed that there was a significant difference in different ethanol concentrations in
extraction with the content of total flavonoids, total phenolics, and β-carotene (p<0.05). The optimal
ethanol solvent for extracting flavonoids, phenolic compounds, and β-carotene from pepino melon
fruit was 70% ethanol.

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	jurnal pharmaciana
Date Deposited:	19 Aug 2023 03:49
Last Modified:	06 Sep 2023 03:53
URI:	http://eprints.uad.ac.id/id/eprint/46478

Actions (login required)

View Item