Agitohutomo, Muhammad and Edityaningrum, Citra Ariani and Utari, Dina and Desvita, Widea Rossi and aristiani, windy and Nurani, Laela Hayu Cytotoxicity of quercetin and curcumin combination against HeLa cells line. [Artikel Dosen]
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Abstract
Curcumin and quercetin combination are potential candidates for anticancer.
Previous studies reported this combination active against several cancer cells
including breast cancer, myeloid leukemia, and melanoma. However, the
activity of this combination against cervical cancer has not been reported, yet.
This study aimed to evaluate the cytotoxicity of the combination of curcumin
and quercetin against HeLa and Vero cells. MTT assay was applied to evaluate
cell growth inhibition. The inhibitory 50% concentration (IC50) of curcumin and
quercetin on HeLa and Vero cells was determined and the selectivity index (SI)
was then calculated. Combination index (CI) was calculated after evaluation
of the cytotoxicity of the curcumin and quercetin combination at various
concentration of 1 IC50, ½ IC50, and ¼ IC50. The IC50 of the curcumin against the
HeLa and Vero cells were 26.57 ± 2.00 μM and 73.89 ± 12.93 μM with a SI of 2.78.
Whereas the IC50 of the quercetin against the HeLa and Vero cells were 149.52
± 21.09 μM and 1094.47 ± 15.68 μM with a SI of 7.32. The CI of combinations of
curcumin and quercetin at concentrations of ¼ IC50 was 0.78 indicating a mildmoderate
synergistic effect, whereas the combination at a concentration of 1
IC50 or ½ IC50, the CI value was >0.9 indicating an antagonistic effect.
| Item Type: | Artikel Dosen |
|---|---|
| Keyword: | cancer; combination; curcumin; HeLa; quercetin |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Divisi / Prodi: | Faculty of Pharmacy (Fakultas Farmasi) > Pharmacy Doc |
| Depositing User: | Laela |
| Date Deposited: | 01 Apr 2024 00:50 |
| Last Modified: | 01 Apr 2024 00:50 |
| URI: | http://eprints.uad.ac.id/id/eprint/61645 |
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