Antioxidant, analgetic, and anti-inflammatory activity test of purple leaf ethanol extract (Graptophyllum pictum L. Griff) in vitro and in vivo

Ocvinta, Sisca and Dewi, Niluh Puspita and Utami, Indah Kurnia and Mul, Darmayanti and Alaydrus, Alaydrus and Anwarudin, Wawang Anwarudin Antioxidant, analgetic, and anti-inflammatory activity test of purple leaf ethanol extract (Graptophyllum pictum L. Griff) in vitro and in vivo. Pharmaciana, 14 (2). ISSN ISSN:2088-4559,e-ISSN:2477-0256

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Abstract

Purple leaves (Graptophyllum pictum L. Griff) are one plant that is widely used as a medicine. Purple
leaves have pharmacological activities that can be used as medicine, including antioxidants, antiinflammatory,
antidiabetic, analgesics, immunomodulators, antihemorrhoids, and antibacterial. The
objective of this study was to determine the antioxidant properties of ethanol extract from purple leaves
as well as its properties as an analgesic and anti-inflammatory against Wistar rats. Antioxidant activity
was measured using the DPPH method with quercetin as a comperation in vitro the in vivo study of 25
Wistar rats was divided into 5 groups consisting of 5 animals each, namely normal control, positive
control, negative control, treatment group with a dose of 50 mg/kg BW and treatment group with a dose
of 100 mg/kg BW. The analgetic activity test was conducted by the writhing test method, while antiinflammatory
activity was measured by measuring the percentage of inflammatory volume using a
mercury plethysmometer. The data was analyzed using the One-way ANOVA SPSS software program.
The results showed that antioxidant activity showed an IC50 value of 72.312 ± 24.406 μg/mL (strong
antioxidant). Meanwhile, in the analgetic test, the highest percentage of analgesic effectiveness was
found at a dose of 100 mg/kg BW with a value of 129.64% and showed anti-inflammatory activity at a
dose of 100 mg/kg BW with a percentage decrease in edem volume of 28.73% at the sixth hour.

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	jurnal pharmaciana
Date Deposited:	07 Aug 2024 01:01
Last Modified:	07 Aug 2024 01:01
URI:	http://eprints.uad.ac.id/id/eprint/65861

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