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Phytochemical screening and antioxidant activity of ethanolic extract and ethyl acetate fraction from basil leaf (Ocimum basilicum L.) by DPPH radical scavenging method

Warsi, M.Sc., Apt. and Adhani, Rachmi Sholichah (2017) Phytochemical screening and antioxidant activity of ethanolic extract and ethyl acetate fraction from basil leaf (Ocimum basilicum L.) by DPPH radical scavenging method. IOP Conference Series: Materials Science and Engineering.

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Abstract

Abstract. Background. Basil leaf (Ocimum basilicum L.) contains various compounds such as flavonoid, alkaloid, phenol and essential oil, so it needs to be fractionated to find out the flavonoid compound with the greatest potential as an antioxidant. Objective.This research was aimed to know the chemical compound, antioxidant potential of ethanolic extract and ethyl acetate fraction of basil leaf. Method. The basil leaf was extracted by maceration using ethanol 70 %. The crude extract was fractionated with ethyl acetate. The ethanolic extract and ethyl acetate fraction were screened of phytochemical content including identification of flavonoid, alkaloid and polyphenol. The antioxidant activity of the ethanolic extract and ethyl acetate fraction were tested qualitatively with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and phosphomolybdate. Its antioxidant activity was determined quantitatively using DPPH radical scavenging method. Results. Phytochemical screening test showed that ethanolic extract and ethyl acetate fraction from basil leaf contain flavonoid, polyphenol, and alkaloid. The qualitativeanalysis of antioxidant activity of ethanolic extract andethyl acetate fraction from basil leaf showed an antioxidant activity. The IC50 value of ethanolic extract, ethyl acetate fraction and quercetin were 1,374.00 ± 6.20; 389.00 ± 1.00; 2.10 ± 0.01μg/mL respectively. Conclusion. The research showed that antioxidant activity of the ethyl acetate fraction more potential than the ethanol extract of the basil leaf, but less than quercetin.

Item Type: Article
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User: Warsi, M.Sc., Apt.
Date Deposited: 02 Oct 2017 04:40
Last Modified: 09 Jan 2018 06:05
URI: http://eprints.uad.ac.id/id/eprint/7510

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