The Screening Of Cytotoxic Fraction From Elephantopus scaber Linn against Human Cervical Cancer (Hela) Cells

Nurkhasanah, Nurkhasanah and Candra Trisnamurti, Kartika and Dewi Gunaryanti, Retty and Widyastuti, Trisna and Listyowati, Yeni (2015) The Screening Of Cytotoxic Fraction From Elephantopus scaber Linn against Human Cervical Cancer (Hela) Cells. International Journal of Pharma Sciences and Research (IJPSR), 6 (6). p. 1011. ISSN 0975-9492

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Abstract

Purpose: Cervical cancer has become the second leading cause of death after breast cancer. Elephantopus
scaber Linn (ES) has been used traditionally for curing various diseases. The objective of this study was to
explore the active fraction from ES as anticancer and the mode of cell death. Method: The ES herb were
extracted using maceration method with ethanol followed by evaporation to get the concentrated extract. The
extract were fractionated using petroleum ether, chloroform, ethyl acetate and methanol respectively. The
cytotoxic activity of were carried out with MTT method, and the mode of cell death were observed by acridine
orange-Ethidium bromide double staining. Result: The result showed that the ES fraction has cytotoxic
activity against HeLa cell lines with IC50 values of petroleum ether, chloroform, ethyl acetate and methanol
fractions were 185; 42.26; 95.72; 650 μg/ml respectively. The mode of cell death showed by the doublestaining
method were apoptosis. The cell dead could be distinguish from live cells. The cell dead appear to absorb the
ethidium bromide as the DNA of the cell membrane was damage and ethidium bromide could interact with
DNA of the cells, while the live cells do not absorb the ethidium bromide as the cell membrane still intact.
Conclusion: The chloroform fraction of ES is the most cytotoxic fraction against HeLa cells.
Keywords: Elephantopus scaber, cytotoxic, IC50, apoptosis.

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi)
Depositing User:	Dr Nurkhasanah Mahfudh
Date Deposited:	12 Oct 2017 05:05
Last Modified:	12 Oct 2017 05:05
URI:	http://eprints.uad.ac.id/id/eprint/7642

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