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THE COMBINATION OF ROSELLA (HIBISCUS SABDARIFFA, L) AND STEVIA (STEVIA REBAUDIANA) EXTRACTS INCREASE THE ANTIOXIDANT ACTIVITY AND STABILITY

Nurkhasanah, Nurkhasanah and Nur Indah Minangsari, Dyah and Aniska Yulianny, Vania (2016) THE COMBINATION OF ROSELLA (HIBISCUS SABDARIFFA, L) AND STEVIA (STEVIA REBAUDIANA) EXTRACTS INCREASE THE ANTIOXIDANT ACTIVITY AND STABILITY. International Journal of Pharmacy and Pharmaceutical Sciences, 8 (5). pp. 411-412. ISSN 0975-1491

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Abstract

Objective: Antioxidant has been widely used for preventing many diseases. The objective of this study was to determine the effect of the combination of rosella (Hibiscus sabdariffa, L) and stevia (Stevia rebaudiana, L) leaf extract on antioxidant activity and stability. Methods: Rosella and stevia were extracted by maceration using ethanol. The combinations of rosella and stevia extract prepared were 0:1; 3:1; 1:1; 1:3; and 1:0. The antioxidant activity was determined using the DPPH method. The antioxidant stability test was carried out by incubate the extract solution on the different temperature of 60,70, and 80 oC and followed by DPPH assay. Results: The results showed that antioxidant value expressed as the IC50 of rosella and stevia extract of 0:1; 3:1; 1:1; 1:3; and 1:0 were 450.32±14.10; 418.8±18.48; 272.9±7.14; 246.39±4.96; and 148.29±3.81 ug/ml respectively. The antioxidant stability expressed as degradation constant (K) of rosella and stevia extract of 0:1; 3:1; 1:1; 1:3; and 1:0 were 1.804.10[24]; 2.825.10[14]; 2.704.105; 2.708.104; 2.089.109; 2.062.102/min. Conclusion: The combination of rosella: stevia 1:3 had the most active antioxidant and the most stable composition. The combination increase the antioxidant activity and stability significantly (P<0.05). Keywords: Hibiscus sabdariffa, Stevia rebaudiana, Antioxidant, DPPH method

Item Type: Article
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi)
Depositing User: Dr Nurkhasanah Mahfudh
Date Deposited: 12 Oct 2017 05:06
Last Modified: 12 Oct 2017 05:06
URI: http://eprints.uad.ac.id/id/eprint/7643

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