Wijaya, Andi and Hayu Nurani, Laela and Nurkhasanah, Nurkhasanah (2014) AKTIVITAS ANTIOKSIDAN SEDIAAN NANOPARTIKEL KITOSAN EKSTRAK ETANOL KELOPAK ROSELA (Hibiscus sabdariffa L) PADA TIKUS HIPERKOLESTEROL : PENGUKURAN KADAR MALONDIALDEHID (MDA). KARTIKA JURNAL ILMIAH FARMASI, 2 (1). pp. 1-6. ISSN 2354-6565
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Abstract
Hypercholesterolemia can induce oxidative stress and are associated with atherosclerosis and other cardiovascular disorders. Administration of a preparation of chitosan nanoparticles roselle extract (NKER) can prevent oxidative stress because it contains flavonoids which have an effect
as an antioxidant anthocyanin. Decreased oxidative stress can be determined by measuring the levels of malondealdehid (MDA) produced by the reaction of lipid peroxidation. The purpose of this study was to determine the effect of administration of a preparation of chitosan nanoparticles roselle calyx ethanol extract of antioxidant activity by measuring the levels of MDA. This study used 25 adult rats of Sprague Dawley strain were divided into 5 groups. The
first group was given a standard feed diet as baseline, group II was induced with pure cholesterol. Group III, IV and V induced pure cholesterol as well as the dosage given NKER with successive doses of 25 mg/kg, 50 mg/kg and 100 mg/KgBB. The treatment was done for 30 days. On day - 31 each rat blood taken for measurement of total cholesterol, as well as the measurement of MDA using thiobarbituric acid reactive substance (TBARS). The results showed an increase in levels of MDA in mice induced pure cholesterol (group II) with a mean concentration of 7.13 ± 0.22 nmol / ml significantly different to the baseline with a mean
concentration of 0.87 ± 0.77 nmol / ml (p≤0,05). The mean levels of MDA in group III, IV and V respectively was 5.49 ± 0.18 nmol / ml; 4.18 ± 0.37 nmol / ml; and 2.18 ± 0.15 nmol / ml,significantly different when compared with the baseline group and the group II (p≤0,05). Decreased levels of MDA in mice given dosage NKER hypercholesterolemia showed that nker
preparations have antioxidant activity, which is owned by the highest antioxidant activity
NKER dosage dose 100mg/KgBB.
Keywords : Antioxidant, Nanoparticles, Roselle, malondialdehyde
| Item Type: | Artikel Umum |
|---|---|
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisi / Prodi: | Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi) |
| Depositing User: | Dr Nurkhasanah Mahfudh |
| Date Deposited: | 12 Oct 2017 05:14 |
| Last Modified: | 12 Oct 2017 05:14 |
| URI: | http://eprints.uad.ac.id/id/eprint/7651 |
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