Enterobacter hormaechei: an endophytic bacterium found in Avocado Peel (Persea americana Mill.) with antioxidant properties

Rustini, Rustini and Rahmi, Khalila and Putra, Purnawan Pontana and Andayani, Regina and Dwinatrana, Khiky (2024) Enterobacter hormaechei: an endophytic bacterium found in Avocado Peel (Persea americana Mill.) with antioxidant properties. Pharmaciana, 14 (3). ISSN ISSN:2088-4559,e-ISSN:2477-0256

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Abstract

Avocado peels (AVP) are renowned for their potent antioxidant properties, making them highly
effective in preventing oxidation and free radical formation. Endophytes, microorganisms residing
within plant tissues, have demonstrated the ability to produce novel compounds with remarkable
biological activities. These bioactive compounds are sometimes even more potent than those found in
their host plants. This study explores the potential of endophytes from avocado peels as rich sources of
antioxidant compounds. AVP samples are collected, surface-sterilized, and segmented before being
cultured in growth media. The bacteria are then isolated, purified, and subjected to ethyl acetate
extraction to evaluate their antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH)
microassay. Samples demonstrating favorable antioxidant properties undergo molecular identification
through 16S rRNA gene sequencing. Four bacterial strains are successfully isolated, with only the APK4
strain exhibiting significant antioxidant activity with an IC50 value of 302.3 μg/mL. Molecular analysis
and phylogenetic tree construction reveal that APK4 is closely related to the Enterobacter hormaechei
species, with a percent identity value of 99.93%. These findings highlight the potential of active
metabolites from endophytic bacteria in AVP extracts as promising lead compounds for the development
of novel drugs, nutraceuticals, and cosmetic ingredients.

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	jurnal pharmaciana
Date Deposited:	19 Apr 2025 07:40
Last Modified:	19 Apr 2025 07:40
URI:	http://eprints.uad.ac.id/id/eprint/83048

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