DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method

Permanasari, Etin Diah and Sunaryo, Hadi and Wirman, Adia Putra and Rahmadini, Nuriza and Aggasy, Savira Yustinah and Nikmatullah, Nurul Azmah (2024) DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method. Pharmaciana, 14 (3). ISSN ISSN:2088-4559,e-ISSN:2477-0256

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Abstract

The meat-based products are highly susceptible to counterfeiting, primarily due to high consumer demand of meat derivative products, such as meatballs. This demand creates opportunities for food fraud by specific industries, including adulterating meat with non-halal species, such as rats. This research aimed to detect rat meat contamination in meatball samples from the Indonesian local market using Real-time Polymerase Chain Reaction (RT-PCR). The RT-PCR amplification involved an initial denaturation step at 95°C for 3 minutes, followed by denaturation at 95°C for 15 seconds, and annealing/extension at 60°C for 1 minute. The rat-specific probe primer included in the kit produced an increasing curve in the External Positive Control (EPC) with a Ct value of 27.22, and no amplification occurred in the Negative Control (NTC). The analysis of 30 samples from meatball vendors yielded negative results, as there was no increase in the FAM (rat) curve, indicating that none of the meatballs were contaminated with rat DNA.

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	jurnal pharmaciana
Date Deposited:	21 Apr 2025 01:28
Last Modified:	21 Apr 2025 01:28
URI:	http://eprints.uad.ac.id/id/eprint/83060

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