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The immunomodulatory effect of Zingiber cassumunar ethanolic extract on phagocytic activity, nitrit oxide and reaxtive oxygen intermediate secretions of macrophage in mice.

Nurkhasanah, Nurkhasanah and Santoso, R D and Fauziah, R The immunomodulatory effect of Zingiber cassumunar ethanolic extract on phagocytic activity, nitrit oxide and reaxtive oxygen intermediate secretions of macrophage in mice. International Pharmacy Conference UAD 2017 on Product Authenticati International Pharmacy Conference UAD 2017 on Product Authentication: Key Factor i. ISSN 1757-899X

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Abstract

Immunomodulators could protect the body from a variety of infectious agents and boost immunity. Zingiber cassumunar rhizome or bangle potentially showed as an immunomodulator through increasing of macrophage activity in vitro. The objective of the study was to determine the effect of Z. cassumunar rhizome ethanolic extract on phagocytic activity, nitrite oxide (NO) and reactive oxygen intermediate (ROI) secretions in macrophages in vivo. A total of 200 g of Z. cassumunar rhizome was powdered, macerated in 96% ethanol and evaporated to get concentrated extract. Mice were divided into 5 groups as follow: the normal group was given by water only, the negative control group was given by a 0.94% CMC-Na suspension, the treatment groups were given by 250, 500 and 1000 mg/kgBW, respectively, of Z. cassumunar ethanolic extract. The extract was administered orally for 7 days. On the 8th day the mice were injected intraperitoneally 0.7 mg/kg BW of lipopolysaccharide. Four hours later macrophage was isolated. Furthermore, the determination of the phagocytic activity, NO and ROI secretions levels of macrophage were performed. The treatments of 250, 500 and 1000 mg/kg BW of Z. cassumunar ethanolic extract significantly increase the ROI and NO secretions levels (p<0.05), but did not increase the phagocytic activity (p>0.05) of macrophage. Z. cassumunar ethanolic extract have immunomodulatory effect in vivo. Keywords: Z.cassumunar, imunomodulator, phagocytic activity, ROI, NO

Item Type: Article
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi)
Depositing User: Dr Nurkhasanah Mahfudh
Date Deposited: 12 Jan 2018 04:24
Last Modified: 12 Jan 2018 04:24
URI: http://eprints.uad.ac.id/id/eprint/8575

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