Black cumin seed oil increases phagocytic activity and secretion of IL-12 by macrophages.

Akrom, Akrom and Mustofa, Mustofa (2017) Black cumin seed oil increases phagocytic activity and secretion of IL-12 by macrophages. Biomedical Research, 28 (12). pp. 5241-5246. ISSN 0970-938X

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Abstract

Interleukin-12 (IL-12) is one of cytokines that regulate the innate and adaptive immune responses
produced by macrophages. This study was to investigate the effect of Black Cumin Seed Oil (BCSO) to
the structure of peripheral blood, phagocytic activity, and secretion of IL-12 by macrophage peritoneal
Sprague-Dawley (SD) rat. The 36 female Sprague Dawley (SD) rats divided into 6 groups. Normal group
was only given standards food and drink. Treatments group were given BCSO 0.25, 2.5 and 5 ml/kg
BW/day for 14 d. Positive control group was given thymoquinone 50 mg/kg BW/day for 14 d. Solvent
control group was given DMSO. Blood test, phagocytic activity and secretion of IL-12 by macrophage in
vitro performed on the 15th day. Latex and NBT assay method were conducted to measure the
phagocytic activity of macrophages. The expression level of IL-12 and TLR4 were analysed by ELISA.
Data were statistically tested by one-way ANOVA with significance 95%. BCSO was increase phagocytic
activity, secretion of IL-12 by macrophage, and the expression of TLR4. The highest phagocytosis
percent (56.83 ± 6.37%) and phagocytosis index (5.18 ± 0.39) was performed by 0.25 BCSO group and
did not significantly different with thymoquinone group. The highest level of IL-12 was in 2.5 BCSO
group (66.33 ± 2.11 µM)

Item Type:	Artikel Umum
Keyword:	nigella sativa; thymoquinone; Il-12; Macrophages; immunomodulator
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	Akrom Akrom
Date Deposited:	22 Jan 2018 07:51
Last Modified:	22 Jan 2018 07:51
URI:	http://eprints.uad.ac.id/id/eprint/8609

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