Repository Universitas Ahmad Dahlan

UJI AKTIVITAS ANTIBAKTERI INFUSA DAUN SIRSAK (Annona muricata L.) SECARA in Vitro TERHADAP Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 35218 SERTA PROFIL KROMATOGRAFI LAPIS TIPISNYA

Downloads

Downloads per month over past year

Sari, Yeni Dianita and Djannah, Sitti Nur and Nurani, Laela Hayu (2010) UJI AKTIVITAS ANTIBAKTERI INFUSA DAUN SIRSAK (Annona muricata L.) SECARA in Vitro TERHADAP Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 35218 SERTA PROFIL KROMATOGRAFI LAPIS TIPISNYA. Fakultas Kesehatan Masyarakat, Universitas Ahmad Dahlan.

[img] Text
10. UJI_AKTIVITAS_ANTIBAKTERI_INFUSA_DAUN_SIRSAK__Anno.1.pdf

Download (23kB)

Abstract

Background: A research has been done to test the In vitro antibacterial activities of sirsak leaf (Annona muricata L.) infuse toward Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 35218 to analyze its thin layer chromatography profile. This research done to observe the antibacterial activity from sirsak leaf infuse. Method: A test on the antibacterial activity was done by using liquid dilution method. The concentration infuse in sterile destilate water using to test the antibacterial activity toward S. aureus were 100%, 95%, 90%, 85%, 80%, and 75% w/v, while toward E. coli were 100%, 90%, 80%, 70%, 60%, and 50% w/v. To detect Chemical contains of the sirsak leaf infuse were identified using Screening method of Phytochemistry and Thin Layer Chromatography (TLC). Result: The result showed that the Minimum Bacterial Concentration (MBC) of sirsak leaf infuse S. aureus were 85% w/v, while E. coli could not be show antibacterial activity until 100% w/v concentration. The Minimum Inhibition Concentration (MIC) could not be identified by the liquid dilution because the mixture were turbid and the colour is dark brown. Screening method of Phytochemistry use tube-test and Thin Layer Chromatography showed that the infuse contain flavonoid, poliphenol, and alkaloid. Conclusion: Infuse of sirsak leaf (Annona muricata L.) has antibacterial activity against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 35218 profil infuse. Thin Layer Chromatography showed that the infuse contain flavonoid, poliphenol, and alkaloid. Keyword: Antibacterial, sirsak leaf, chromatography

Item Type: Other
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi)
Depositing User: Unnamed user with email laelafarmasi@yahoo.com
Date Deposited: 07 Mar 2018 06:58
Last Modified: 07 Mar 2018 06:58
URI: http://eprints.uad.ac.id/id/eprint/8949

Actions (login required)

View Item View Item

Repository Universitas Ahmad Dahlan is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.