Effect of Temulawak (Curcuma xanthorrhiza Roxb.) extract on the MDA Levels and GPx The Brains of Trimethyltin Induced dementia Model Rats

Yuliani, Sapto and Prasetya, Didik Yuni and Bachri, Moch. Saiful (2017) Effect of Temulawak (Curcuma xanthorrhiza Roxb.) extract on the MDA Levels and GPx The Brains of Trimethyltin Induced dementia Model Rats. Advanced Science Letters, 23 (12). pp. 12451-12454.

Text ASL.pdf Download (112kB)

Abstract

Oxidative stress in the brain plays an important role in the pathogenesis of dementia. The rhizome of temulawak
(Curcuma xanthorrhiza Roxb.) contains curcumin and xanthorrhizol which possess antioxidant activity and thus
has the potential to deter oxidative stress. This study aims to identify the effect of the ethanol extract of a
temulawak rhizome on the malondialdehyde (MDA) levels and glutathione peroxides (GPx) activity in the brains
of trimethyltin (TMT) induced dementia model rats. The study employed 30 adult male Sprague Dawley rats
divided into six groups, each consisting of five rats. The normal group was given a CMC-Na 1% solution
orally, the TMT group also received a CMC-Na 1% solution orally, while the treatment groups were orally given
temulawak rhizome extract (TRE) with doses of 50 (E50), 100 (E100) and 150 mg/kg bw (E150), and 200 mg/kg
bw of citicoline (Cit200), respectively. Before treatment, all groups, except the normal group, were injected with
single doses of 8 mg/kg bw of TMT intraperitoneally. The treatment was administered for 21 days. On the 29th
day the rats were sacrificed, their brains were retrieved and had their hemispherium cerebri separated for the
measurement of the MDA levels and GPx activity in its homogenate. Data of the MDA levels and GPx activity
were statistically analyzed with a one-way ANOVA test followed by a posthoc LSD test. Results showed that
TMT injection could raise the MDA levels and lower GPx activity (p < 0�05). The administration of TRE with a
dose of 50 mg/kg bw was able to inhibit increase in MDA levels and augment GPx activity in the brain but had
significant difference from the normal group (p < 0�05). Meanwhile, the administration of 100 and 150 mg/kg
bw doses of TRE could prevent rise in MDA levels and enhance GPx activity of the brain (p < 0�05) with no
meaningful difference from the normal group and the group given citicoline (p > 0�05). It can be concluded from
these results that TRE in doses of 100 and 150 mg/kg bw can avert oxidative stress in TMT induced dementia
model rats.
Keywords: Curcuma xanthorrhiza Roxb., Trimethyltin, MDA, GPx, Dementia

Item Type:	Artikel Umum
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	Dr.drh Sapto Yuliani,MP
Date Deposited:	06 Nov 2019 02:41
Last Modified:	06 Nov 2019 02:41
URI:	http://eprints.uad.ac.id/id/eprint/15590

Actions (login required)

View Item