Similarity Check: Validation Of Analytical Method For Determining Ciprofloxacin’s Level In Blood Using High Performace Liquid Chromatography (HPLC)

Rahmatullah, Satrio Wibowo and Sandi, Dita Ayulia Dwi and Perwitasari, Dyah Aryani and Darmawan, Endang Similarity Check: Validation Of Analytical Method For Determining Ciprofloxacin’s Level In Blood Using High Performace Liquid Chromatography (HPLC). RJPBCS RESEARCH JOURNAL PHARMACEUTICAL, BIOLOGICAL & CHEMICAL SCIENCES.

Text Validation_Of_Analytical_Method_For_Determining_Ci.pdf Download (905kB)

Abstract

Ciprofloxacin is a broad-spectrum antibiotic, often used empirically for infection. Ciprofloxacin is an
antibiotic whose activity depends on levels in blood (concentration depends), potential effect of ciprofloxacin
will be optimal when ciprofloxacin’s levels in blood is high compared with its MIC. To measure the level of
ciprofloxacin in the patient's blood required a simple and accurate method of analysis. This study aims to
validation of analysis method for determining level of ciprofloxacin. This study use of mobile phase 2% acetic
acid and acetonitrile (84: 16). HPLC conditions include HPLC devices, YMC C18 column (4,6 x 150 mm: 5 μm),
UV detector 280 nm and flow rate is 1,0 mL / min. Validation parameter in this study are selectivity, accuracy,
linearity, LOD and LOQ. The results of analysis method for measure levels of ciprofloxacin is selective (α >
1,9177) at time 3,845 minute. The HPLC method of ciprofloxacin meets the acceptability criteria of %recovery
value is 96,9%. Eligible precision test % RSD is <3,5%. The value of r
2 on the linearity is 0.9989. LOD value is 1,1
μg/mL and LOQ value is 3,6 μg/mL.
Keywords: ciprofloxacin, HPLC, validation

Item Type:	Other
Subjects:	Z Bibliography. Library Science. Information Resources > ZA Information resources > ZA4050 Electronic information resources
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > Program of Professional Pharmacist
Depositing User:	Dr.rer.nat Endang Darmawan
Date Deposited:	14 May 2020 14:39
Last Modified:	14 May 2020 14:39
URI:	http://eprints.uad.ac.id/id/eprint/18720

Actions (login required)

View Item