In vitro activity of immunomodulator of N-Hexane fraction of bangle (Zingiber cassumunar Roxb.) Ethanol extract

Nurkhasanah, Nurkhasanah and Sulistyani, Nanik and Noorlina, Noorlina (2019) In vitro activity of immunomodulator of N-Hexane fraction of bangle (Zingiber cassumunar Roxb.) Ethanol extract. [Artikel Dosen]

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Abstract

Zingiber cassumunar is potentially an immunomodulator. The aim of study is to determine the immunomodulatory activity of n-hexane fraction of Zingiber cassumunar ethanol extracts with a measurement of phagocytic activity and lymphocyte proliferation in vitro. Macrophage cells are isolated from peritoneum of mice. Cells were planted and added to latex and n-hexane fraction of Zingiber cassumunar ethanol extract and then painted using 20% giemsa. Lymphocyte cells are isolated from lymph. Cells were planted and given n-hexane fraction of Zingiber cassumunar ethanol extract. Incubate for 3 days using 5% CO2 incubator at 37°C. Data were analysed using SPSS with a confidence level of 95%. The results of the average percentage of phagocytic activity and phagocytic index of n-hexane fraction of Zingiber cassumunar ethanol extract of 0.025 μg/ml, 0.050 μg/ml, 0.100 μg/ml and controls were respectively 5.247% and 5.311%, 7.475% and 7.720%, 10.593% and 11.393% and 15.989% and 19.317%. The results of the average lymphocyte proliferation absorbance of 0.025 μg/ml, 0.050 μg/ml, 0.100 μg/ml and controls respectively are 0.107, 0.127, 0.126 and 0.149. The n-hexane fraction of Zingiber cassumunar ethanol extract can reduce the phagocytic activity of macrophages (p<0.05) and lymphocyte proliferation (p<0.05) in mice in vitro.

Item Type:	Artikel Dosen
Subjects:	Q Science > Q Science (General) Q Science > QR Microbiology R Medicine > R Medicine (General) R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > Program of Professional Pharmacist
Depositing User:	Dr Nanik Sulistyani
Date Deposited:	23 Sep 2020 14:39
Last Modified:	23 Sep 2020 14:39
URI:	http://eprints.uad.ac.id/id/eprint/20568

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