Korespondensi Antibacterial Activity and TLC-Bioautgraphy Analysis of the Active Fractions of Muntingia calabura L. Leaves Against Staphylococcus aureus

Mawardi, Rizky H. and Sulistyani, Nanik and Nurkhasanah, Nurkhasanah and Desyratnaputri, Ricke (2020) Korespondensi Antibacterial Activity and TLC-Bioautgraphy Analysis of the Active Fractions of Muntingia calabura L. Leaves Against Staphylococcus aureus. Fakultas Farmasi, Universitas Sanata Dharma.

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Abstract

This study aims to determine the antibacterial activity and TLC-bioautography profile of the active fractions of Muntingia calabura L. leaves against Staphylococcus aureus. Muntingia calabura L. leaves were macerated with ethanol 96% then fractionated with n-hexane, ethyl acetate, and methanol solvent, respectively. The antibacterial activity was tested by the Kirby-Bauer method to determine the most active fraction and the lowest concentration that inhibited the growth of Staphylococcus aureus. TLC-bioautography was tested using chloroform: ethyl acetate (2:8) as the mobile phase and silica gel F254 as the stationary phase. Antibacterial activity test of n-hexane and ethyl acetate fractions at a concentration of 10% w/v showed activities with inhibition zone diameter of 0.33±0.288 and 9.66±5.77 mm, respectively. At the same time, the methanol fraction showed no activity. The lowest concentration of ethyl acetate fraction which still showed the inhibition zone was 0.312% w/v. The TLC-bioautography profile showed active spots with an Rf value of 0.82 and had an inhibitory zone diameter of 4.013±0.864 mm. It can be concluded that the ethyl acetate fraction was the most active fraction that inhibited the growth of Staphylococcus aureus and had one active spot on the bioautography test.

Item Type: Other
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
R Medicine > R Medicine (General)
R Medicine > RS Pharmacy and materia medica
Divisi / Prodi: Faculty of Pharmacy (Fakultas Farmasi) > Program of Professional Pharmacist
Depositing User: Dr Nanik Sulistyani
Date Deposited: 21 Jan 2021 04:18
Last Modified: 21 Jan 2021 04:18
URI: http://eprints.uad.ac.id/id/eprint/22035

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