The Effect of Inoculum Concentration on the Growth Profile and Antibacterial Activity of Actinomycete (A6K) Isolates

Ningsih, Ika Maylani and Sulistyani, Nanik (2017) The Effect of Inoculum Concentration on the Growth Profile and Antibacterial Activity of Actinomycete (A6K) Isolates. [Artikel Dosen]

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Abstract

Actinomycetes are important microorganisms renowned for their ability to produce secondary metabolites as antibiotics. A6K Actinomycetes were isolated from papaya plant soil and yielded antibacterial metabolites. This research aims to identify the effect of inoculum concentration on the growth profile and antibacterial activity of A6K isolates as producers of antibiotics. The bacterial concentration within the inoculum (CFU/mL) was determined by a turbidity test using visible spectrophotometry on inoculum 10%, 20%, 30% v/v. The growth profile of A6K isolates was made with a correlation graph between incubation time and weight of biomass in the liquid cultures of A6K isolates, while the temporal profile of secondary metabolites was established with a graph correlating incubation time and growth inhibition zones of S. aureus and E. coli. Statistical analysis by repeated ANOVA was performed to verify the influence of inoculum concentration rate and incubation period on inhibition zone and biomass weight. According to the growth profile graph, the stationary phase occurred after the fourth day of incubation. Results revealed that inoculum concentration and incubation period gave a significant difference to the resulting inhibition zones. The higher the concentration of inoculum, the larger the bacterial content and the shorter the incubation time needed to obtain maximum antibacterial activity

Item Type: Artikel Dosen
Subjects: Q Science > QR Microbiology
R Medicine > R Medicine (General)
R Medicine > RS Pharmacy and materia medica
Divisi / Prodi: Faculty of Pharmacy (Fakultas Farmasi) > Pharmacy Doc
Depositing User: Dr Nanik Sulistyani
Date Deposited: 06 Jul 2021 09:09
Last Modified: 06 Jul 2021 09:09
URI: http://eprints.uad.ac.id/id/eprint/25663

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