Shi, Yanan and Prabakusuma, Adhita Sri and Zhao, Qiong and Wang, Xuefeng and Huang, Aixiang (2019) Proteomic analysis of Moringa oleifera Lam. leaf extract provides insights into milk-clotting proteases. [Artikel Dosen]
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Abstract
As a new food source, Moringa oleifera Lam. leaves containing large amounts of proteins attract increasing attention. This study detected and characterized hydrolytic and milk-clotting enzymes in these leaves. The proteins were examined by proteomics. A total of 3378 proteins were identified, mostly comprising enzymes of carbohydrate and protein metabolism, including 676 hydrolases, 548 oxidoreductases, glycoside hydrolases and proteinases. A serine/threonine-endopeptidase with a molecular mass of 56.146 kDa and an isoelectric point of 5.27 with milk-clotting activity was obtained from M. oleifera leaves by multistage ultrafiltration and anion exchange chromatography, and characterized by ESI mass spectrometry. The milk-clotting activity was inhibited over 95% by chymostatin, which confirmed its chymotrypsin-like serine protease nature. The protease had a milk-clotting activity/proteolytic activity (MCA/PA) activity ratio of 126.76, an optimal pH of 8.0, and an optimal temperature of 65 °C, indicating its potential use for cheese production and in food industry.
| Item Type: | Artikel Dosen |
|---|---|
| Subjects: | Q Science > Q Science (General) S Agriculture > S Agriculture (General) T Technology > T Technology (General) Z Bibliography. Library Science. Information Resources > ZA Information resources |
| Divisi / Prodi: | Faculty of Economics (Fakultas Ekonomi) > S1-Food Servicee Bussines (S1-Bisnis Jasa Makanan) |
| Depositing User: | Dr. agr. Adhita Sri Prabakusuma |
| Date Deposited: | 30 Oct 2023 09:23 |
| Last Modified: | 30 Oct 2023 09:23 |
| URI: | http://eprints.uad.ac.id/id/eprint/51314 |
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