Peer Review : ANTIANGIOGENIC EFFECTS OF WATER FRACTION OF GREEN ALGAE (Spirogyra sp.) ETHANOL EXTRACT WITH CHORIO ALLANTOIC MEMBRANE METHOD

Suryaningrum, Ketty and Widyaningsih, Wahyu Peer Review : ANTIANGIOGENIC EFFECTS OF WATER FRACTION OF GREEN ALGAE (Spirogyra sp.) ETHANOL EXTRACT WITH CHORIO ALLANTOIC MEMBRANE METHOD. Proceeding of International Conference on Drug Development of Natural Resources.

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Abstract

Background. Angiogenesis plays an important role in tumor progression. Therapeutic
angiogenesis is a major focus in cancer treatment. Modern treatment of cancer currently has
alternative to inhibit cancer through antiangiogenesis process, it is expected to inhibit the formation
of new blood vessels around the tumor, the supply of nutrients and oxygen by the blood to the tumor
can be inhibited. This study aims to determine the fraction of water antiangiogenesis effect of ethanol
extract of green algae (Spirogyra sp.) by using the method of chorio allantoic membrane (CAM).
Methods. In this study subject were devided into 7 groups; group I of the control disc, group II
on the disc+PBS control group, group III of the control disc+PBS +bFGF, variation in the
concentration of the water fraction of ethanol extract of green algae (Spirogyra sp.) is group IV, V,
VI, VII which is each of these group has variation of doses 100, 200, 400 and 800 μg/ml. CAM is
obtained from embryonated chicken eggs aged 8-9 days, then it is given treatment bFGF
(angiogenesis inductor) and water fractions of ethanol extract of green algae (Spirogyra sp.), and
then incubated for 3 days.
Results and Discussion. Observations are made in the macroscopic to see the inhibitory
activity of blood vessels from the water fraction of ethanol extract of green algae (Spirogyra sp.).
Having obtained the result of macroscopic observation, the data of the number of blood vessels were
tested statistically by the method of Mann Whitney and Kruskal Wallis with 95% confidence level.
Conclusions. The results showed that the water fraction of ethanol extract of green algae with
doses 100, 200, 400 and 800 μg/ml can inhibit angiogenesis of embryonated chicken eggs compared
to bFGF control. In conclusion, the water fraction of ethanol extract of green algae has
antiangiogenesis activity in chorio allantoic membrane

Item Type:	Other
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	Dr, M.Si.. Wahyu Widyaningsih
Date Deposited:	09 Apr 2019 06:07
Last Modified:	09 Apr 2019 06:07
URI:	http://eprints.uad.ac.id/id/eprint/13629

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