Determination of epms content and anti-inflammatory test rhizome extract Kaempferia galanga, L by inhibition of protein denaturation method

Hayu Nurani, Laela and Asahi, Aris and Susanti, Hari (2020) Determination of epms content and anti-inflammatory test rhizome extract Kaempferia galanga, L by inhibition of protein denaturation method. Pharmaciana, 10 (3). pp. 381-390.

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Official URL: http://journal.uad.ac.id/index.php/PHARMACIANA/art...

Abstract

Kencur (Kempferia galanga, L) is one of the rhizomes that is often used as a constituent component in traditional medicinal formulas. One of the kencur's pharmacological activities is anti-inflammatory. The active compound as an anti-inflammatory is ethyl p-methoxycinnamate (EPMC). This study aims to determine the content and ascertainment of EPMC compounds and the anti-inflammatory activity of kencur rhizome extract. Kencur extract can be obtained by the maceration method using 96% ethanol solvent. The EPMC content determination was held using the TLC-densitometry method, while the EPMC compounds were confirmed using the GC MS method. The anti-inflammatory activity test was done using inhibiting protein denaturation methods. The extract results obtained 12.67% yield. The result of EPMC content in kencur extract was 10.05 ± 4.57. The GCMS kencur extract results showed an abundance of EPMC compounds at a retention time of 7.088 with a peak area of 72290779. The results of IC50 anti-inflammatory for the standard substance (EPMC) were 5.306 ± 5.028. The results of IC50 anti-inflammatory the sample (kencur extract) was 303.487 ± 1.201. Ethanol extract of kencur contains ethyl p-methoxycinnamate (EPMC) and has anti-inflammatory activity by inhibiting protein denaturation.

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	jurnal pharmaciana
Date Deposited:	25 Sep 2021 04:07
Last Modified:	25 Sep 2021 04:07
URI:	http://eprints.uad.ac.id/id/eprint/28451

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