Peer Review Comparison of Specthrophotometric and TLC-Densitometric Technique in Determination of Phytomelatonin in Green Algae (Sprirogyra Sp) Ethanolic Extract

Susanti, Hari and Widyaningsih, Wahyu and Salamah, Nina and Fertaveni, Beta Zudia and Puspitasari, Efi (2013) Peer Review Comparison of Specthrophotometric and TLC-Densitometric Technique in Determination of Phytomelatonin in Green Algae (Sprirogyra Sp) Ethanolic Extract. [Artikel Dosen]

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Peer Review - Susanti - phytomelatonin green algae aethanolic extract spectrophotometry TLC densitometry.pdf
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Abstract

Background. Instrumental analysis method give a difference measurement results in
determination of content level of active substance in medicinal herb.
Objective. The aim of this study is to campare the level content of phytomelatonin in green
algae extract determinated by spectrophotometry and TLC-densitometry technique.
Methods. The phytomelatonin was extracted from green algae using 96% aethanol. The
qualitative Alkaloid screening were done by using Mayer and Dragendorff test. The quantitative
determination of phytomelatonin were done spectrophotometrically at 277nm wavelength and using
TLC densitometry technique with silica gel GF 254 plate and eluated by BAW ( n-buthanol: acetic
acid: water=12:3:5 v/v). The spots were scanned at 254nm wavelength
Outcome measured. Phytomelatonin level in aethanolic extract of green algae
Results. The results showed that the content phytomelonin content of green algae level by
spectrophotometric technique was 0.22±0.01 % and 0.88±0.04 % was assayed by TLC-densitometric.
Conclusion. The TLC-densitometry technique gave the higher phytomelatonin of green algae
level than specthrophotometry technique (p<0.05)

Item Type:	Artikel Dosen
Subjects:	Q Science > Q Science (General) R Medicine > R Medicine (General) T Technology > T Technology (General)
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > Pharmacy Doc
Depositing User:	Mrs Hari Susanti
Date Deposited:	21 May 2022 01:17
Last Modified:	21 May 2022 01:17
URI:	http://eprints.uad.ac.id/id/eprint/32374

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