Hasil cek similarity" Ethanolic extract of the natural product of Daun sirih (Piper betle) leaves may impede the effectiveness of the plasma jet contact style for acute wounds"

Setyowati, Heni and Rahayu, Esti and Nasruddin, Nasruddin and Nurani, Laela Hayu and Darmawati, Sri and Rohmani, Afiana and Lutfiyati, Heni and Wahyuningtyas, Eka Sakti and Sikumbang, Isabella Meliawati and Muhlisin, Zaenul and Sukeksi, Andri and Nuroini, Fitri (2019) Hasil cek similarity" Ethanolic extract of the natural product of Daun sirih (Piper betle) leaves may impede the effectiveness of the plasma jet contact style for acute wounds". Clinical Plasma Medicine. ISSN 2212-8166

Text HASIL CEK_Plasma medicine, ROS, RNS, PAW, Natural product, Wound.pdf Download (3MB)

Abstract

Purpose: An investigation was carried out to determine the effect of an ethanolic extract of the natural product of
Daun sirih or Piper betle leaves on the effectiveness of plasma jet treatment for cutaneous acute wound healing in
a small animal model mimicking a clinical setting.
Method: An atmospheric plasma jet using medical grade argon gas as a carrier gas was developed. The ethanolic
extract of Piper betle leaf (EPB) was formulated. Optical emission spectroscopy and chemical methods were
applied to evaluate the presence of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the gas
phase and in aqueous and ethanolic media. Small animals were classified into 5 groups, namely, Control (C),
Plasma jet (P), Ethanolic extract of Piper betle leaf (EPB), Plasma jet followed by EPB (P-EPB) and EPB followed
by plasma jet (EPB-P). The contact and meander styles of plasma jet treatment for wounds were applied daily on
acute wounds for 1 min, either alone or before or after EPB treatments. Visual evaluation of wounds was conducted for 14 days. Microscopic evaluation was conducted on days 7, 11 and 14. General staining, namely,
haematoxylin-eosin and Azan staining, was conducted to evaluate neoepithelialisation and new collagen formation.
Results: This research showed that wound healing in the P group was faster than that in the other groups, while
that in groups containing EPB was the same as that in C. In the P group, the number of days to reach peak
inflammation was the fewest. On day 7, neoepithelialisation and new collagen formation in P were significantly
higher than those in other groups.
Conclusion: Plasma jet treatment alone is able to promote inflammation, neoepithelialisation and new collagen
formation to accelerate acute wound healing; however, its admixture with EPB may impede such effectiveness.
Based on the characterization of the ROS and RNS results, the ethanol solvent may play a primary role in
impeding its effectiveness

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi)
Depositing User:	Laela
Date Deposited:	27 Oct 2022 05:06
Last Modified:	27 Oct 2022 05:06
URI:	http://eprints.uad.ac.id/id/eprint/36973

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