Protection Effect of Self-Nanoemulsifying Drug Delivery System (SNEDDS) Piroxicam as Ulcerogenic Agent towards Malondialdehid Level and Protein Expression of Caspase-3, COX-1, COX-2 at Rat Gastric

Wahyuningsih, Iis and Ambarwati, Kurnia and Hapsari, Erninda Ayu and Ikhsanudin, Azis and Vanda Pertiwi, Deasy and Widyaningsih, Wahyu (2020) Protection Effect of Self-Nanoemulsifying Drug Delivery System (SNEDDS) Piroxicam as Ulcerogenic Agent towards Malondialdehid Level and Protein Expression of Caspase-3, COX-1, COX-2 at Rat Gastric. Journal of Food and Pharmaceutical Sciences (J. Food Pharm. Sci), 8 (2). pp. 275-285. ISSN pISSN = 2089-7200, eISSN = 2339-0948

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Abstract

Piroxicam can damage the gastric mucosa through two main mechanisms, topical and systemic.
Topical mucosal damage occurs because the piroxicam is lipophilic and acidic, making it easier for hydrogen
ions to enter the mucosa and cause ulcers. The aim of this study was to determine the protection effect of
SNEDDS piroxicam ulcerogenic agent against malondialdehyde (MDA) level and protein expression of caspase�3, COX-1, COX-2. The research was conducted using the test animals as much as 30 male white Sprague dawley
(SD) rats aged 1-2 months with a weight of 100-200 grams divided into 5 groups consisting of Normal Control,
Trigger Control, Drug Control, Carrier Control and Treatment Control. Treatment was given for 28 days orally.
On the 29th day blood samples were also taken for the determination of MDA (Malondialdehid) levels by
Thiobarbituric Acid Reactive Substance (TBARs) method using a visible spectrophotometer. Rats were
sacrificed, then gastric organs were taken for immunohistochemical testing of caspase-3 and COX-1 expression,
COX-2. The statistical analysis showed that the piroxicam SNEDDS group and the piroxicam suspension group
decreased expression of the caspase-3 protein, increased COX-1 expression, decreased COX-2 and significantly
decreased MDA levels. The piroxicam-containing SNEDDS (Self-Nanoemulsifying Drug Delivery System) form
has protection effect against ulcogenic piroxicam by decreasing the caspase-3, MDA and COX-2 levels

Item Type:	Artikel Umum
Subjects:	Q Science > Q Science (General)
Depositing User:	Mr Azis Ikhsanudin
Date Deposited:	06 Apr 2023 01:41
Last Modified:	06 Apr 2023 01:43
URI:	http://eprints.uad.ac.id/id/eprint/42154

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