Chlorogenic acid in preventing and curing ultraviolet-induced damage in human skin fibroblast as an antiaging cell model

Girsang, Ermi and Lister, I Nyoman Ehrich and Ginting, Chrismis Novilda and Widowati, Wahyu and Arumwardana, Seila and Marthania, Meganita and Rizal, Rizal Chlorogenic acid in preventing and curing ultraviolet-induced damage in human skin fibroblast as an antiaging cell model. Pharmaciana, 13 (2). p. 2023. ISSN ISSN:2088-4559,e-ISSN:2477-0256

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Abstract

Continuous ultraviolet (UV) irradiation stimulates the over-production of reactive oxygen species
(ROS) to cause degenerative diseases. Chlorogenic acid (CA) is found as plants antioxidant that
promises medicinal effects. This study examined CA protection against UV-damage in human skin
fibroblast (BJ) cells both for curative and preventive therapy. BJ cells were exposed to UV radiation and
the addition of CA (6.26-100 μg/mL) by preventive and curative addition methods. The cells viability
analysis was conducted employing MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-
2-(4-sulfophenyl)-2H-tetrazolium) assay. CA treatment before UV exposure exhibited an increased
percentage of viability cells than the positive control. In detail, the series of CA concentration (6.25,
12.5, and 25 μg/mL) significantly enhanced the percentage of viable cells. The addition of CA after UV
exposure denoted the same results. Furthermore, the lower CA concentrations used, the higher cell
viability resulted. CA at dose 6.25 μg/mL showed the highest viability in cells, while CA 100 μg/mL
resulted in the lowest viability. In short, CA can preserve and treat cells from UV exposure. The outcome
suggested prevention and curative on UV-induced BJ cells, and the tested concentration is applicable
for further experiments.

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	jurnal pharmaciana
Date Deposited:	19 Aug 2023 03:49
Last Modified:	19 Aug 2023 03:49
URI:	http://eprints.uad.ac.id/id/eprint/46423

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