Gingerol and shogaol on red ginger rhizome (Zingiber officinale var. Rubrum) using high-performance liquid chromatography

Sukweenadhi, Johan and Damitasari, Putu Diah and Kartini, Kartini and Christanti, Pissa and Putri, Evanie Noor (2023) Gingerol and shogaol on red ginger rhizome (Zingiber officinale var. Rubrum) using high-performance liquid chromatography. Pharmaciana, 13 (2). pp. 166-178. ISSN ISSN:2088-4559,e-ISSN:2477-0256

Text 3.Sukweenadhi_166– 178.pdf Download (798kB)

Abstract

Red ginger (Zingiber officinale var. Rubrum) is one of the traditional medicines of the Zingiberaceae
family, which contains phenolic ketone compounds, including gingerol and shogaol. At high
temperatures, gingerol compounds become unstable and will change into shogaol. This study optimized
conditions for the simultaneous separation of 6-gingerol, 8-gingerol, 10-gingerol and 6-shogaol from
the red ginger extract using the High-Performance Liquid Chromatography method. The analytical
conditions consisted of five different methods by modifying the composition of the mobile phase, the
elution system, the flow rate of the mobile phase and the optimum UV wavelength. The best conditions
for the simultaneous separation using a ratio of water: acetonitrile mobile phase in method 4 with a
gradient elution system including 0 min (65:35); 1.5 min (40:60); 5-6.5 min (10:90); 7.5–9 min (0:100);
9.5–12 min (65:35); the mobile phase flow rate was 1.1 mL/min at an optimum wavelength of 230 nm
with a retention time of 6-gingerol compound, 6-shogaol, 8-gingerol and 10-gingerol respectively were
4.947; 6.168; 6.554; and 7.412 min and its resolution were 2.267; 1.575; 1.315; and 2.215. Then the
tailings factor and asymmetry values were obtained with an average value of ± 1.

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	jurnal pharmaciana
Date Deposited:	19 Aug 2023 03:49
Last Modified:	19 Aug 2023 03:49
URI:	http://eprints.uad.ac.id/id/eprint/46425

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