Fibrotic effect of monoculture, conditioned media and coculture of fibroblast NIH3T3 cells on collagen secretion and mRNA TGF-beta expressions

Awaluddin, Rizki and Nugrahaningsih, Dwi Aris Agung and Solikhah, Eti Nurwening and Prasetyastuti, Prasetyastuti (2023) Fibrotic effect of monoculture, conditioned media and coculture of fibroblast NIH3T3 cells on collagen secretion and mRNA TGF-beta expressions. Pharmaciana, 13 (3). pp. 179-184. ISSN ISSN:2088-4559,e-ISSN:2477-0256

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Abstract

Fibrosis is common profound in diabetes condition, they characterized by accumulation of
extracellular matrix in tissue then cause tissue injury. The study aim to investigate effect of monoculture,
conditioned media, and coculture fibroblast of NIH3T3 cells (NIH3T3 cells) in LPS-high glucose media
(HG-LPS media) conditions on mRNA TGF-β and collagen secretion. The fibroblast NIH3T3 were
cultured with various culture type there is mono culture NIH3T3 cells, conditioned media from
macrophage RAW264.7 cells culture media, and coculture with RAW264.7 cells (ratio 1:1 and 1:2) in
normal media (media containing 4500 μg/mL of glucose) and HG-LPS media (media containing LPS
0.25 μg/mL and D-glucose 5400 μg/mL). Our findings shows that the TGF-β mRNA expression in RAW
264.7 and NIH 3T3 cell coculture media with a culture ratio of 1:1 (Coculture 1:1) were the highest
(p<0.05) compared than monoculture of NIH3T3 cells (cultured in normal media or HG-LPS Media),
conditioned media from RAW264.7 cells in normal media (CM-M0 Media), and the RAW 264.7 and
NIH 3T3 co-culture cells with 1:2 cultured ratio in HG-LPS Media (Coculture 1:2). On other hand, the
the relative ratio of collagen secretion on Coculture 1:1 were the highest compared to CM-M0 media
(p<0.05). Our study conclude that RAW 264.7 and NIH 3T3 coculture cells with a (1:1) cultured ratio
in LPS and high glucose media (Coculture 1:1) is appropriate for diabetic fibrosis in vitro models.

Item Type:	Artikel Umum
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User:	jurnal pharmaciana
Date Deposited:	19 Aug 2023 03:49
Last Modified:	24 Aug 2023 06:57
URI:	http://eprints.uad.ac.id/id/eprint/46426

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