Wulandari, Dita and Puri, Putri Ratna and Kurnia W, Ratna and Nurkhasanah, Nurkhasanah (2014) Efek Immunostimulan Ekstrak Kelopak Bunga Rosella. Jurnal Farmasi Indonesia, 7 (1). pp. 7-12. ISSN 1412-1107
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Abstract
Theaims of this study was to determine the immunostimulant potential ethanolic extract of roselle catalyx through the proliferation of lymphocytes and phagocytosis ability of macrophages Balb/c mice in vitro. Dried calyx of roselle were extracted using 96% ethanol by maceration method is further polyphenols assay are qualitatively.Vitro test using with immunostimulants are seeing an increase in lymphocyte proliferation using the MTT method andphagocytosis activity macrophages with latex beads. Lymphocyte proliferation is expressed by the difference in absorbance values between trearment and control groups, where as the ability of phagocytosis is expressed as an index of phagocytosis and the SFA. Concentration of extract used was 10-1000 µg/mL. Ethanolic extract of roselle calyc containing polyphenols with total polyphenol concent (TPC) of 2.757%. Ethanol extract of roselle calyx enhanced immune response at law concentrations. Concentrations of 100µg/mL is the optimal concentrations that increased proliferaion of lymphocytest. The highest index of phagocytosis and SFA are at concentrations of 150µg/mL and 250 µg/mL. It canbe conluded that ethanolic extract of roselle calyx has potential as immunostimulants.
keywords: roselle, immune responce, macrophage phagocytosis, lymphocyte proliferation, immunostimulant.
| Item Type: | Artikel Umum |
|---|---|
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisi / Prodi: | Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi) |
| Depositing User: | Dr Nurkhasanah Mahfudh |
| Date Deposited: | 08 Sep 2017 03:50 |
| Last Modified: | 08 Sep 2017 03:50 |
| URI: | http://eprints.uad.ac.id/id/eprint/7269 |
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