Effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-transferase activity in rats

Nurkhasanah, Nurkhasanah and Hayu Nurani, Laela and Rahman Hakim, Zainur (2017) Effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-transferase activity in rats. Tropical Journal of Pharmaceutical Research, 16 (10). pp. 2411-2416. ISSN 1596-9827

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Abstract

Purpose: To determine the effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-trasferase
(GST) activity and its hepatoprotective effect.
Methods: A total of 25 rats were divided randomly into 5 groups (5 rats per group). Group I served as
the baseline, group II was the negative control group, while groups III, IV and V were treated with rosella
extract at doses of 10, 50 and 100 mg /kg /day, respectively for 35 days. On day 36, the animals were
given a single dose of dimethyl benz(a)anthracene (DMBA) orally. After one week, blood was taken
from the sinus orbitalis for measurement of serum glutamate pyruvate transaminase (SGPT) and serum
glutamate oxaloacetate transaminase (SGOT) activities by a kinetic method with DIasys® kit. The
activity of GST was measured in liver homogenate using 1-choloro-2,4-dinitrobenzene (CDNB) method,
while the expression of GST gene was determined by reverse transcriptase polymerase chain reaction
(RT-PCR).
Results: Treatment with rosella extract at 10, 50 and 100 mg /kg for 35 days led to significant increases
in GST activity relative to the control group. In addition, serum SGPT and SGOT activities were
significantly decreased. There were significant increases in the expression of GST gene as evidenced
by increased GST band intensity.
Conclusion: These results indicate that rosella possesses significant hepatoprotective effect against
hepatic injury caused by DMBA treatment. Thus, rosella may be useful for the prevention of oxidative
stress caused by free radicals produced from pollutants and foods.
Keywords: Hibiscus sabdariffa, Rosella, Glutamate pyruvate transaminase (SGPT), Glutamate
oxaloacetate transaminase (SGOT), glutathion-S-transferase (GST)

Item Type:	Artikel Umum
Additional Information:	di aprove oleh Zulfa
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi)
Depositing User:	Dr Nurkhasanah Mahfudh
Date Deposited:	17 Nov 2017 02:26
Last Modified:	17 Nov 2017 02:26
URI:	http://eprints.uad.ac.id/id/eprint/7995

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