CEK SIMILARITY "ISOLATION AND LARVACIDAL ACTIVITY OF ESSENTIAL OIL MANGO TURMERIC RHIZOMES (Curcuma mangga, Val.) AGAINST LARVAE OF Aedes aegypti MOSQUITOES ALSO GAS CHROMATOGRAM-MASS SPECTROMETRY PROFILE"

Lolita, Lolita and Djannah, Sitti Nur and Nurani, Laela Hayu (2012) CEK SIMILARITY "ISOLATION AND LARVACIDAL ACTIVITY OF ESSENTIAL OIL MANGO TURMERIC RHIZOMES (Curcuma mangga, Val.) AGAINST LARVAE OF Aedes aegypti MOSQUITOES ALSO GAS CHROMATOGRAM-MASS SPECTROMETRY PROFILE". Fakultas Farmasi, Universitas Ahmad Dahlan, Proseding ICDDNR.

Text 2.a.1. ISOLATION_AND_LARVACIDAL_ACTIVITY_OF_ESSENTIAL_OIL.pdf Download (2MB)

Abstract

Background. Curcuma mangga.,Val has been recognized by society as traditional drug for a long time. The active ingredience of this plant especially the volatile oil contains monoterpenes and sesquiterpenes. This research aim to know about the larvicide's activity from the volatile oil of Curcuma mangga., Val rhizome and also to analyse its chemical compounds using the GC-MS.
Method. The volatile oil was isolated from Curcuma mangga, Val with steam and water
distillation. The test method for larvicide was done by dissolving the substances into water and added
Tween 20 10 % v/v to the the volatile oil. The consentration of the volatile oil of Curcuma
mangga,Val rhizome used by these are 100 ppm, 150 ppm, 200 ppm, 250 ppm, 300 ppm, 350 ppm. The
concentration of positive control "abate" are 0,01 ppm; 0,025 ppm; 0,05 ppm; 0,1 ppm; 0,5 ppm
while as the negative control is Tween 20 solution. Larvae of Aedes aegypti amount of 10 larvaes for
the each consentration. However, the perception time during 24 hours.
Result. Data of larvae's died were used to estimate the values of LC 50 with the probit analysis
method. The chemical compound of Curcuma mangga., Val can be analysed by the GC-MS. The
results of research showed that the volatile oil of Curcuma mangga, Val. have clear-brass colour,
bitter taste, typically aromatic like mango, rendemen equal to (1,23 ± 0,029)% v/b and refractive
index 1,4881. The value of LC50 for the volatile oil of Curcuma mangga, Val. rhizome is (216,17
±12,51) ppm while abate equal to (0,072 ± 0,024) ppm. This matter indicate that abate more potent to
larvae of Aedes aegypti. The result of analyse the component of the volatile oil of Curcuma mangga,
Val., by the GC-MS obtained of 30 peak chromatogram and six peak which has identified showed the
possibility the existence of alpha-pinene, camphene, beta-pinene, beta-myrcene, eukalyptole,
ar-turmerone.
Key words : larvacidal activity, GC-MS, Curcuma mangga, Aedes aygypti mosquitoes

Item Type:	Other
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisi / Prodi:	Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi)
Depositing User:	Laela
Date Deposited:	07 Mar 2018 06:49
Last Modified:	08 Nov 2019 01:31
URI:	http://eprints.uad.ac.id/id/eprint/8964

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