Nurani, Laela Hayu and Widyarini, Sitarina and Mursyidi, Achmad and Rachmadhaniah, Rachmadhaniah and Emelda, Emelda and Breni, Breni and Ana, Ana CEK SIMILARITY" CO-CHEMOTHERAPY TEST OF ETHYL ACETATE FRACTION OF PASAK BUMI ROOTS EXTRACT (Eurycoma longifolia Jack.) AGAINST T47D CELLS WHICH EXPOSED BY DOXORUBICIN". Fakultas Farmasi,UAD, Proseding International Conference on Herbal Medicine Industrilization as Complementary Teraphy in Natural Disasters.
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Abstract
Background: In an effort to cancer treatment, failure oftenoccurs primarily through chemotherapy, due to the reduce defficacy of chemotherapeutic agents because of the
multi-drug resistance mechanisms, including the doxorubicin. Addition to suppress cancer cell doxorubicines also have an effect on normal cells. Additionally, doxorubicin tends to
cause resistance cell against to drug. Some natural products research begin to be directed for co-chemotherapy agent with the aims for increase sensitivity, supress resistance of cancer cells and decrease of side effect posed by chemotherapy agent.
Objective: The pasak bumi roots have great potential for anticancer and cochemotherapy for breast cancer. Therefore, the pasak bumi roots (Eurycoma Longifolia
Jack.) can be combined with doxorubicin for drug of choice in breast cancer.
Outcome: IC50
Methods: the method of this study will be performed by in vitro test. Amount of 500 g of
pasak bumi root were macerated with ethanol 70 % during 24 hours and remacerated for
3 times. Fractination of extract was use ethyl acetate and aquadest. Cytotoxic test and
combination test in T47D cells were used by MTT method.The concentrations of Ethyl
acetate fraction used 2000 μg, 1000, 500, 250, 125, 62,5 and 31,125 μg/ml with
incubation time 24 hours. And then, observed using Elisa plate reader in 595 nm.
Absorbance readings were then converted into a percentage of the life of the cell to the
IC50values calculated.
Result: IC50 values from ethyl acetate fraction of the pasak bumi roots obtained was 340
μg/ml. The concentration of ethyl acetate fraction whichwas used for combination test
were 340, 255, 170, 85 μg/ml. The combination index was 0,23682.
Conclussion: This result indicate that combination of pasak bumi roots and doxorubicin
have strong synegristic effect with dose selection for pasak bumi roots of 255 μg/ml and
doxorubicin of 0,00193 μg/ml.
Keywords : Breast Cancer T47D, Doxorubicin, Ethyl acetate fraction of pasak bumi
roots, co-chemotherapy
| Item Type: | Other |
|---|---|
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisi / Prodi: | Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi) |
| Depositing User: | Laela |
| Date Deposited: | 07 Mar 2018 06:47 |
| Last Modified: | 08 Nov 2019 01:32 |
| URI: | http://eprints.uad.ac.id/id/eprint/8968 |
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