Hakim, Zainur Rahman and Nurkhasanah, Nurkhasanah and Nurani, Laela Hayu CEK SIMILARITY "THE EFFECT OF ROSELLE ( Hibiscus Sabdariffa L.) CALYX ETHANOLIC EXTRACT ON GST ACTIVITY AND DECREASING OF ALT AND AST LEVEL ON 7,12 DIMETHYL BENZ(A) ANTRASEN (DMBA) INDUCED RATS". Fakultas Farmasi,UAD.
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Abstract
Background : Oxidants or free radicals caused all abnormalities in this human body and
to make liver cells damaged. Various natural products have been observing for drugs or
compounds as antioxidants as Roselle ( Hibiscus sabdariffa L ).
Objective: The aim of this study was to determine ethanolic extract of roselle protect
Method: Roselle was extracted by maceration 60 %ethanol with a ratio of dry calyx and
solvents are (1:3) stirer for 24 hours , then is filtered and concentrated by Rotary
evaporator at 60°C . Maserate was transferred into a porcelain cup and evaporated on
water bath at 60°C . Condensed extract is then stored in a sealed container and be
identified .Forty-five Male Sprague Dawley rats age of 4 weeks divided into 9 groups:
group I are baseline, group II and III was DMBA control group at day 7 and 35 (Single
dose 75 mg/kgbw) . Group IV , V and VI are roselle extract treatment groups with
variations each dose group of 10 mg / kg , 50 mg/kg and 100 mg/kgbw on day 7 induced
DMBA 75 mg/kgbw. Group VII , VIII and IX treated with roselle extract in dose variation of
each group 10 mg/kgbw, 50 mg/kgbw and 100 mg/kgbw. On day 35 induced DMBA 75
mg/kg BB . After that on day 7 and 35 after DMBA single dose, they were fasted (total 5
test animals each group) for 16 hours, then collect blood from eye (orbital plexus) and
observed the SGOT and SGPT activity . After that, the animal were sacrified and hepar
organ were observe GST gene expression by RT-PCR method. The data were analyzed
using Kruska-wallis, Mann-Whitney, ANOVA, Least Significant Difference (LSD). Content
of Polyfenol and Flavonoids use gallic acid and quercetin standart.
Outcome Measured : -
Result&conclusion: Results showed the increasing of ALT level (32.63±0.72) and AST
level (27.09±1.51) at DMBA group compared to baseline (p<0.05). Significant differences
occurred ALT and AST activity to DMBA control compared (p<0.05), decreasing of ALT
and AST activity in a group of 10, 50, 100mg/kg/day compared to DMBA control group, a
significant decrease of AST activity 18.45±0.92 (p<0.05) in the dose of 100 mg/kg/day
Keywords : Antioxidants , Roselle , glutathione peroxidase ( GPx ) , SGPT SGOT
activity, GST activity, SD rats , DMBA
| Item Type: | Other |
|---|---|
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Divisi / Prodi: | Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi) |
| Depositing User: | Laela |
| Date Deposited: | 06 Mar 2018 03:06 |
| Last Modified: | 11 Nov 2019 06:56 |
| URI: | http://eprints.uad.ac.id/id/eprint/8969 |
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