Morphological studies of apoptotic Hela cells death induced by eurycomanone

Mahfudh, Nurkhasanah Morphological studies of apoptotic Hela cells death induced by eurycomanone. Majalah Farmasi Indonesia.

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Eurycomanone is a cytotoxic ingredient found in Eurycoma longifolia Jack.
Previous studies have noted its activity against many epithelial cell lines. In this
study, eurycomanone had obvious cytotoxic effect on HeLa cells by methylene
blue staining assays. After HeLa cells were treated with eurycomanone, typical
morphological changes, including cytoplasm shrinkage and decrease of cell
volume were observed by light microscope. Chromatin condensation and nuclear
fragmentation could be observed by fluorescence microscope after staining with
Hoechst 33258 nuclear staining. DNA fragmentation and apoptotic body
formation that is characteristic of apoptosis could be determined after treated
cells were assayed with TUNEL (terminal deoxynucleotidyl transferase mediated
d-UTP nick end labeling). The externalization of phosphatidyl serine (PS) could
be determined by flow cytometry analysis using Annexin-V/PI double staining.
The result suggested that eurycomanone exerted antiproliferative activity on
HeLa cells by inducing apoptosis

Item Type: Artikel Umum
Subjects: R Medicine > RS Pharmacy and materia medica
Divisi / Prodi: Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi)
Depositing User: Dr Nurkhasanah Mahfudh
Date Deposited: 10 Jul 2012 04:29
Last Modified: 10 Jul 2012 04:29

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