Anti Angionesis Activity Of Ethanol Extract Of Green Algae (Spyrogyra sp) Purified With Chorio Allantoic Membrane (CAM) Method

Widyaningsih, Wahyu and Salamah, Nina and Susanti, Hari (2013) Anti Angionesis Activity Of Ethanol Extract Of Green Algae (Spyrogyra sp) Purified With Chorio Allantoic Membrane (CAM) Method. Proceeding The International Conference On Safety Management Of Central Cytotoxic Reconstitution In Pharmacy Practice. pp. 115-121. ISSN 978-979-18458-6-1

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Background. Angiogenesis plays an important role in tumor progression. Modern treatment of cancer currently has an alternative way to inhibit cancer through antiangiogenesis process.It is expected to inhibit the formation of new blood vessels around the tumor, the supply of nutrients and oxygen by the blood to the tumor can be inhibited.
Objective. This study aims to determine the antiangiogenesis activity of ethanol extract of green algae (Sprogyra Sp)Purified by using the method of chorio allantoic membrane(CAM).
Methods. In this study used 8 groups: Group I was a control that content only paper discs,paper discs contain a group II + PBS, group III contain paper disc +PBS+bFGF,group IV,V,VI,VII,VIII as a treating were given 10,20,40,160,and 320 ug/ml of ethanol exctrac of green algae respectively. CAM is obtained from embryonated chicken eggs aged 8 days. Incubated for 3 days afterward.The maccroscopic observation is used to see the inhibitory activity of blood vessels.Statictical analysis used Kruskal-Wallis test to compare among each other Significantly, continuated by Mann Whtney test (P<0.05).
Outcome measured. Inhibitoty activity (IC50)
Results.The results showed that the ethanol extract of green algae purified with concentration 10ug/ml (p=0:03) can inhibit angiogenesis of embryoned chicken eggs Comparated to bFGF control.
Couclusion. In conclusion, the ethanol extract of green algae has antiangiogenesis activity in chorio allantoic membrane.

Keywords: antiangiogenesis, CAM,green algaee, Spyrogyra Sp

Item Type: Artikel Umum
Subjects: R Medicine > RS Pharmacy and materia medica
Divisi / Prodi: Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User: Ms Nina Salamah
Date Deposited: 21 Oct 2015 03:13
Last Modified: 21 Oct 2015 03:13

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