Antioxicant Activity as Free Radical Scavenger Of Green Algae (Spirogyra sp.) Ethanolic Extract Using DPPH method

Salamah, Nina and Widyaningsih, Wahyu and Izati, Innayah (2013) Antioxicant Activity as Free Radical Scavenger Of Green Algae (Spirogyra sp.) Ethanolic Extract Using DPPH method. The first PSU and UAD Joint Seminar On Pharmaceutical Sciencies " Building Collaboration on Herbal Medicine Innovation".

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Antioxidants are compoundthat can inhibit the oxidation reaction, to scavenge free Radicals and highly reactive molecules. Green algae is the largest division Consisting of more than 500 genera and about 15,000 species.Spirogyra sp is an example of species that lives in freshwater. This study aims to Determine the antioxidant activity as scavenger free Radicals of the ethanol exctract of green algae Spirogyra sp by using DPPH(1,1-diphenyl-2-pikrilhdrazil).
Materials and Methods:
Ethanol exctract prepared from the power of green algae Spirogyra sp. By maceration method.Trials of antioxidant activity in DPPH visible spectrophotometry. Gallic acid used as a positive control.The free radiacls scavenging expressed with ES50 . Based on the data, ES50 ethanol exctract of Spirogyra sp and gallic acid were analyzed with SPSS 16.0 correlation.
The result showed that ethanolic exctract os Spirogyra sp.have activity as scavenger free radical. The ES50 value of Spirogyra sp. was (92,83±0,58)µg/ml and then the positive control, gallic acid was (0,73±0,04) µg/ml .
Based on statistical analys, the ES50 value of gallic acid and the ethanolic extract of Spirogyra sp.are significantly difference.
Keyword: Spirogyra sp, green algae, gallic acid, DPPH, antioxidant

Item Type: Artikel Umum
Subjects: R Medicine > RS Pharmacy and materia medica
Divisi / Prodi: Faculty of Pharmacy (Fakultas Farmasi) > S1-Pharmacy (S1-Farmasi)
Depositing User: Ms Nina Salamah
Date Deposited: 28 Oct 2015 03:06
Last Modified: 28 Oct 2015 03:06

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