The cytotoxic effect of Elephantopus scaber Linn extract against breast cancer (T47D) cells

Sulistyani, Nanik and Nurkhasanah, Nurkhasanah The cytotoxic effect of Elephantopus scaber Linn extract against breast cancer (T47D) cells. International Pharmacy Conference UAD 2017 on Product Authentication: Key Factor in Quality Control of Pharmaceutical Products. pp. 1-5. ISSN 1757-899X

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Breast cancer is one of the main cause of death. Elephantopus scaber Linn (ES)
which has been used as a traditional medicine contains an antitumor compounds. This study
aimed to explore the active fraction from ethanolic extract of ES as anticancer and to determine
its inhibition effect on the cell proliferation cycle of breast cancer (T47D) cells. The ES leaf
was macerated with ethanol and then evaporated to get the concentrated extract. The extract
was fractionated using petroleum ether, chloroform, and methanol respectively. The cytotoxic
activity of each fraction was carried out with MTT method, and the inhibition of cell cycle test
were observed by flowcytometry method. The result showed that ES and the fractions have
cytotoxic activity against T47D cell lines with IC50 values of extract, petroleum ether,
chloroform, and methanol fractions were 58.36±2.38, 132.17±9.69, 7.08±2.11, and
572.89±69.23 µg/mL. The inhibition effect of ethanol extract on the lifecycle of cells was
occured in sub G1 phase. There was no prolonging of G1, S, G2/M and polyploidy phase of
T47D cell lines. The chloroform fraction of ES is the most cytotoxic fraction against T47D
cells without prolonging the cell lifecycle.
Keywords: Elephantopus scaber, cytotoxic, T47D, IC50, cell cy

Item Type: Artikel Umum
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica
Divisi / Prodi: Faculty of Pharmacy (Fakultas Farmasi) > S2-Master of Pharmacy (S2-Farmasi)
Depositing User: Dr Nurkhasanah Mahfudh
Date Deposited: 05 Jan 2018 07:46
Last Modified: 05 Jan 2018 07:46

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